biostudies-literatureUnknown2(5)Korrodi-Gregorio LReversible phosphorylation plays an important role as a mechanism of intracellular control in eukaryotes. PPP1, a major eukaryotic Ser/Thr-protein phosphatase, acquires its specificity by interacting with different protein regulators, also known as PPP1 interacting proteins (PIPs). In the present work we characterized a physiologically relevant PIP in testis. Using a yeast two-hybrid screen with a human testis cDNA library, we identified a novel PIP of PPP1CC2 isoform, the T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) that has recently been described as a Tctex1 dynein light chain family member. The overlay assays confirm that TCTEX1D4 interacts with the different spliced isoforms of PPP1CC. Also, the binding domain occurs in the N-terminus, where a consensus PPP1 binBiology open453-65https://www.ebi.ac.uk/biostudies/studies/S-EPMC3654263biostudies-literatureTCTEX1D4, a novel protein phosphatase 1 interactor: connecting the phosphatase to the microtubule network.PMC3654263Brauns AKLuers GFardilha MEsteves SLda Cruz E Silva EFEsteves PJda Cruz E Silva OAKorrodi-Gregorio LFreitas MJVieira SISilva JVAbrantes JfalseTCTEX1D4, a novel protein phosphatase 1 interactor: connecting the phosphatase to the microtubule network.Reversible phosphorylation plays an important role as a mechanism of intracellular control in eukaryotes. PPP1, a major eukaryotic Ser/Thr-protein phosphatase, acquires its specificity by interacting with different protein regulators, also known as PPP1 interacting proteins (PIPs). In the present work we characterized a physiologically relevant PIP in testis. Using a yeast two-hybrid screen with a human testis cDNA library, we identified a novel PIP of PPP1CC2 isoform, the T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) that has recently been described as a Tctex1 dynein light chain family member. The overlay assays confirm that TCTEX1D4 interacts with the different spliced isoforms of PPP1CC. Also, the binding domain occurs in the N-terminus, where a consensus PPP1 bin2013-01-01T00:00:00Z2013 May2026-05-03T13:42:00.938Z2026-04-07T19:14:38.085ZS-EPMC36542632378909310.1242/bio.20131065