{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(1)"],"submitter":["Kolotilin I"],"pubmed_abstract":["<h4>Background</h4>Chloroplast transformation in tobacco has been used extensively to produce recombinant proteins and enzymes. Chloroplast expression cassettes can be designed with different configurations of the cis-acting elements that govern foreign gene expression. With the aim to optimize production of recombinant hemicellulases in transplastomic tobacco, we developed a set of cassettes that incorporate elements known to facilitate protein expression in chloroplasts and examined expression and accumulation of a bacterial xylanase XynA. Biomass production is another important factor in achieving sustainable and high-volume production of cellulolytic enzymes. Therefore, we compared productivity of two tobacco cultivars - a low-alkaloid and a high-biomass - as transplastomic expression "],"journal":["Biotechnology for biofuels"],"pagination":["65"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3655837"],"repository":["biostudies-literature"],"pubmed_title":["Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields."],"pmcid":["PMC3655837"],"pubmed_authors":["Menassa R","Kolotilin I","Pereira EO","Kaldis A","Laberge S"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields.","description":"<h4>Background</h4>Chloroplast transformation in tobacco has been used extensively to produce recombinant proteins and enzymes. Chloroplast expression cassettes can be designed with different configurations of the cis-acting elements that govern foreign gene expression. With the aim to optimize production of recombinant hemicellulases in transplastomic tobacco, we developed a set of cassettes that incorporate elements known to facilitate protein expression in chloroplasts and examined expression and accumulation of a bacterial xylanase XynA. Biomass production is another important factor in achieving sustainable and high-volume production of cellulolytic enzymes. Therefore, we compared productivity of two tobacco cultivars - a low-alkaloid and a high-biomass - as transplastomic expression ","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 May","modification":"2026-05-06T21:48:24.655Z","creation":"2026-04-07T22:23:06.535Z"},"accession":"S-EPMC3655837","cross_references":{"pubmed":["23642171"],"doi":["10.1186/1754-6834-6-65"]}}