{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["110(20)"],"submitter":["Barber JS"],"funding":["Intramural NIH HHS"],"pubmed_abstract":["The ability of T-cells to respond to foreign antigens and to appropriately regulate this response is crucial for maintaining immune homeostasis. Using combinatorial peptide libraries, we functionally measured broad T-cell reactivity and observed impaired reactivity in established models of T-cell receptor repertoire restriction and in previously unrecognized disease contexts. By concurrently analyzing T-regulatory and T-effector cells, we show strong functional correlation between these subsets in healthy individuals and, strikingly, that alterations of this balance are associated with T helper type 2 (Th2)-mediated disease in a lymphopenic setting. Finally, we demonstrate that peptide-based priming of polyclonal naive cells with relatively low concentrations skews toward Th2 differentiati"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["8164-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3657790"],"repository":["biostudies-literature"],"pubmed_title":["Peptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases."],"pmcid":["PMC3657790"],"pubmed_authors":["Sheikh V","Yokomizo LK","Garabedian E","Sokolic R","Freeman AF","Milner JD","Barber JS","Sereti I","van Dijk E","Candotti F","Weng NP"],"additional_accession":[]},"is_claimable":false,"name":"Peptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases.","description":"The ability of T-cells to respond to foreign antigens and to appropriately regulate this response is crucial for maintaining immune homeostasis. Using combinatorial peptide libraries, we functionally measured broad T-cell reactivity and observed impaired reactivity in established models of T-cell receptor repertoire restriction and in previously unrecognized disease contexts. By concurrently analyzing T-regulatory and T-effector cells, we show strong functional correlation between these subsets in healthy individuals and, strikingly, that alterations of this balance are associated with T helper type 2 (Th2)-mediated disease in a lymphopenic setting. Finally, we demonstrate that peptide-based priming of polyclonal naive cells with relatively low concentrations skews toward Th2 differentiati","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 May","modification":"2025-04-19T15:20:40.868Z","creation":"2019-03-27T01:10:08Z"},"accession":"S-EPMC3657790","cross_references":{"pubmed":["23637345"],"doi":["10.1073/pnas.1302103110"]}}