biostudies-literatureUnknown110(20)Barber JSIntramural NIH HHSThe ability of T-cells to respond to foreign antigens and to appropriately regulate this response is crucial for maintaining immune homeostasis. Using combinatorial peptide libraries, we functionally measured broad T-cell reactivity and observed impaired reactivity in established models of T-cell receptor repertoire restriction and in previously unrecognized disease contexts. By concurrently analyzing T-regulatory and T-effector cells, we show strong functional correlation between these subsets in healthy individuals and, strikingly, that alterations of this balance are associated with T helper type 2 (Th2)-mediated disease in a lymphopenic setting. Finally, we demonstrate that peptide-based priming of polyclonal naive cells with relatively low concentrations skews toward Th2 differentiatiProceedings of the National Academy of Sciences of the United States of America8164-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC3657790biostudies-literaturePeptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases.PMC3657790Sheikh VYokomizo LKGarabedian ESokolic RFreeman AFMilner JDBarber JSSereti Ivan Dijk ECandotti FWeng NPfalsePeptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases.The ability of T-cells to respond to foreign antigens and to appropriately regulate this response is crucial for maintaining immune homeostasis. Using combinatorial peptide libraries, we functionally measured broad T-cell reactivity and observed impaired reactivity in established models of T-cell receptor repertoire restriction and in previously unrecognized disease contexts. By concurrently analyzing T-regulatory and T-effector cells, we show strong functional correlation between these subsets in healthy individuals and, strikingly, that alterations of this balance are associated with T helper type 2 (Th2)-mediated disease in a lymphopenic setting. Finally, we demonstrate that peptide-based priming of polyclonal naive cells with relatively low concentrations skews toward Th2 differentiati2013-01-01T00:00:00Z2013 May2025-04-19T15:20:40.868Z2019-03-27T01:10:08ZS-EPMC36577902363734510.1073/pnas.1302103110