{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["51(4)"],"submitter":["Lu X"],"pubmed_abstract":["Outbreaks of human adenovirus (HAdV) acute respiratory illness (ARI) have been well documented among civilians and unvaccinated military recruits. Among the 7 recognized HAdV species (A to G), species B (particularly serotypes 3, 7, 11, 14, and 21) and E (serotype 4) have more often been associated with epidemic ARI. Rapid detection and type-specific identification of these viruses would enhance outbreak response and help guide prevention and control measures. To this end, we developed type-specific real-time quantitative PCR (qPCR) assays for HAdV types 3, 4, 7, 11, 14, 16, and 21 targeting the HAdV hexon gene. All type-specific qPCR assays reproducibly detected as few as 10 copies/reaction of quantified hexon recombinant plasmids with a linear dynamic range of 8 log units (10(1) to 10(8)"],"journal":["Journal of clinical microbiology"],"pagination":["1089-93"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3666809"],"repository":["biostudies-literature"],"pubmed_title":["Quantitative real-time PCR assay panel for detection and type-specific identification of epidemic respiratory human adenoviruses."],"pmcid":["PMC3666809"],"pubmed_authors":["Trujillo-Lopez E","Lu X","Lott L","Erdman DD"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative real-time PCR assay panel for detection and type-specific identification of epidemic respiratory human adenoviruses.","description":"Outbreaks of human adenovirus (HAdV) acute respiratory illness (ARI) have been well documented among civilians and unvaccinated military recruits. Among the 7 recognized HAdV species (A to G), species B (particularly serotypes 3, 7, 11, 14, and 21) and E (serotype 4) have more often been associated with epidemic ARI. Rapid detection and type-specific identification of these viruses would enhance outbreak response and help guide prevention and control measures. To this end, we developed type-specific real-time quantitative PCR (qPCR) assays for HAdV types 3, 4, 7, 11, 14, 16, and 21 targeting the HAdV hexon gene. All type-specific qPCR assays reproducibly detected as few as 10 copies/reaction of quantified hexon recombinant plasmids with a linear dynamic range of 8 log units (10(1) to 10(8)","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Apr","modification":"2025-04-04T23:39:41.897Z","creation":"2019-03-27T01:10:32Z"},"accession":"S-EPMC3666809","cross_references":{"pubmed":["23325827"],"doi":["10.1128/jcm.03297-12","10.1128/JCM.03297-12"]}}