biostudies-literatureUnknown288(23)Kugler JEThe nuclei of most vertebrate cells contain members of the high mobility group N (HMGN) protein family, which bind specifically to nucleosome core particles and affect chromatin structure and function, including transcription. Here, we study the biological role of this protein family by systematic analysis of phenotypes and tissue transcription profiles in mice lacking functional HMGN variants. Phenotypic analysis of Hmgn1(tm1/tm1), Hmgn3(tm1/tm1), and Hmgn5(tm1/tm1) mice and their wild type littermates with a battery of standardized tests uncovered variant-specific abnormalities. Gene expression analysis of four different tissues in each of the Hmgn(tm1/tm1) lines reveals very little overlap between genes affected by specific variants in different tissues. Pathway analysis reveals that loThe Journal of biological chemistry16690-16703https://www.ebi.ac.uk/biostudies/studies/S-EPMC3675603biostudies-literatureHigh mobility group N proteins modulate the fidelity of the cellular transcriptional profile in a tissue- and variant-specific manner.PMC3675603Bohla ABecker LHuang DHorsch MOllert MGailus-Durner VAdler TWolf ERathkolb BPrehn CFurusawa TKugler JEBusch DHRacz IEickelberg OYildirim AOHrabe de Angelis MStoger TAguilar-Pimentel JAHolter SMGarfinkel BWurst WOvcharenko IBustin MOrly JAdamski JGarrett LFuchs HRochman MBeckers JZimmer AKlopstock TfalseHigh mobility group N proteins modulate the fidelity of the cellular transcriptional profile in a tissue- and variant-specific manner.The nuclei of most vertebrate cells contain members of the high mobility group N (HMGN) protein family, which bind specifically to nucleosome core particles and affect chromatin structure and function, including transcription. Here, we study the biological role of this protein family by systematic analysis of phenotypes and tissue transcription profiles in mice lacking functional HMGN variants. Phenotypic analysis of Hmgn1(tm1/tm1), Hmgn3(tm1/tm1), and Hmgn5(tm1/tm1) mice and their wild type littermates with a battery of standardized tests uncovered variant-specific abnormalities. Gene expression analysis of four different tissues in each of the Hmgn(tm1/tm1) lines reveals very little overlap between genes affected by specific variants in different tissues. Pathway analysis reveals that lo2013-01-01T00:00:00Z2013 Jun2025-04-05T00:36:08.696Z2019-06-05T18:43:25ZS-EPMC36756032362059110.1074/jbc.m113.46331510.1074/jbc.M113.463315