biostudies-literatureImami KCanadian Institutes of Health Research1632-43https://www.ebi.ac.uk/biostudies/studies/S-EPMC3675819biostudies-literatureUnknown12(6)During the late stages of infection, Salmonella secretes numerous effectors through a type III secretion system that is encoded within Salmonella pathogenicity island 2 (SPI2). Despite the importance of SPI2 as a major virulence factor leading to the systemic spread of the bacteria and diseases, a global view of its effects on host responses is still lacking. Here, we measured global impacts of SPI2 effectors on the host phosphorylation and protein expression levels in RAW264.7 and in HeLa cells, as macrophage and nonphagocytic models of infection. We observe that SPI2 effectors differentially modulate the host phosphoproteome and cellular processes (e.g. protein trafficking, cytoskeletal regulation, and immune signaling) in a host cell-dependent manner. Our unbiased approach reveals the iMolecular & cellular proteomics : MCPGlobal impact of Salmonella pathogenicity island 2-secreted effectors on the host phosphoproteome.PMC3675819MOP-10551MOP-77688Bhavsar APImami KRogers LDFoster LJYu HFinlay BBBrown NFfalseGlobal impact of Salmonella pathogenicity island 2-secreted effectors on the host phosphoproteome.During the late stages of infection, Salmonella secretes numerous effectors through a type III secretion system that is encoded within Salmonella pathogenicity island 2 (SPI2). Despite the importance of SPI2 as a major virulence factor leading to the systemic spread of the bacteria and diseases, a global view of its effects on host responses is still lacking. Here, we measured global impacts of SPI2 effectors on the host phosphorylation and protein expression levels in RAW264.7 and in HeLa cells, as macrophage and nonphagocytic models of infection. We observe that SPI2 effectors differentially modulate the host phosphoproteome and cellular processes (e.g. protein trafficking, cytoskeletal regulation, and immune signaling) in a host cell-dependent manner. Our unbiased approach reveals the i2013-01-01T00:00:00Z2013 Jun2026-05-30T14:05:55.733Z2026-04-08T07:25:09.13ZS-EPMC36758192345999110.1074/mcp.m112.02616110.1074/mcp.M112.026161