biostudies-literatureUnknown87(12)Mao QPlant reoviruses are thought to replicate and assemble within cytoplasmic, nonmembranous structures called viroplasms. Here, we established continuous cell cultures of the white-backed planthopper (Sogatella furcifera Horváth) to investigate the mechanisms for the genesis and maturation of the viroplasm induced by Southern rice black-streaked dwarf virus (SRBSDV), a fijivirus in the family Reoviridae, during infection of its insect vector. Electron and confocal microscopy revealed that the viroplasm consisted of a granular region, where viral RNAs and nonstructural proteins P6 and P9-1 accumulated, and a filamentous region, where viral RNAs, progeny cores, viral particles, as well as nonstructural proteins P5 and P6 accumulated. Our results suggested that the filamentous viroplasm matrix wJournal of virology6819-28https://www.ebi.ac.uk/biostudies/studies/S-EPMC3676135biostudies-literatureNew model for the genesis and maturation of viroplasms induced by fijiviruses in insect vector cells.PMC3676135Han QChen QJia DMao QWei TZheng SMa YChen HfalseNew model for the genesis and maturation of viroplasms induced by fijiviruses in insect vector cells.Plant reoviruses are thought to replicate and assemble within cytoplasmic, nonmembranous structures called viroplasms. Here, we established continuous cell cultures of the white-backed planthopper (Sogatella furcifera Horváth) to investigate the mechanisms for the genesis and maturation of the viroplasm induced by Southern rice black-streaked dwarf virus (SRBSDV), a fijivirus in the family Reoviridae, during infection of its insect vector. Electron and confocal microscopy revealed that the viroplasm consisted of a granular region, where viral RNAs and nonstructural proteins P6 and P9-1 accumulated, and a filamentous region, where viral RNAs, progeny cores, viral particles, as well as nonstructural proteins P5 and P6 accumulated. Our results suggested that the filamentous viroplasm matrix w2013-01-01T00:00:00Z2013 Jun2025-04-19T16:45:32.553Z2021-02-19T23:53:26ZS-EPMC36761352357649910.1128/JVI.00409-1310.1128/jvi.00409-13