{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Najm FJ"],"funding":["NEI NIH HHS","NIMH NIH HHS","NINDS NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["426-33"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3678540"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(5)"],"pubmed_abstract":["Cell-based therapies for myelin disorders, such as multiple sclerosis and leukodystrophies, require technologies to generate functional oligodendrocyte progenitor cells. Here we describe direct conversion of mouse embryonic and lung fibroblasts to induced oligodendrocyte progenitor cells (iOPCs) using sets of either eight or three defined transcription factors. iOPCs exhibit a bipolar morphology and global gene expression profile consistent with bona fide OPCs. They can be expanded in vitro for at least five passages while retaining the ability to differentiate into multiprocessed oligodendrocytes. When transplanted to hypomyelinated mice, iOPCs are capable of ensheathing host axons and generating compact myelin. Lineage conversion of somatic cells to expandable iOPCs provides a strategy t"],"journal":["Nature biotechnology"],"pubmed_title":["Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells."],"pmcid":["PMC3678540"],"funding_grant_id":["K08 EY019880","F31NS083354","R33 MH087877","R01 NS030800","R21 MH087877","T32GM008056","T32 GM008056","MH087877","P30CA043703","NS30800","P30 CA043703","F31 NS083354","EY019880"],"pubmed_authors":["Lager AM","Factor DC","Karl RT","Maeda T","Tesar PJ","Caprariello AV","Najm FJ","Zaremba A","Miller RH","Wyatt K"],"additional_accession":[]},"is_claimable":false,"name":"Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.","description":"Cell-based therapies for myelin disorders, such as multiple sclerosis and leukodystrophies, require technologies to generate functional oligodendrocyte progenitor cells. Here we describe direct conversion of mouse embryonic and lung fibroblasts to induced oligodendrocyte progenitor cells (iOPCs) using sets of either eight or three defined transcription factors. iOPCs exhibit a bipolar morphology and global gene expression profile consistent with bona fide OPCs. They can be expanded in vitro for at least five passages while retaining the ability to differentiate into multiprocessed oligodendrocytes. When transplanted to hypomyelinated mice, iOPCs are capable of ensheathing host axons and generating compact myelin. Lineage conversion of somatic cells to expandable iOPCs provides a strategy t","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 May","modification":"2026-05-30T06:57:12.223Z","creation":"2026-04-08T06:43:58.783Z"},"accession":"S-EPMC3678540","cross_references":{"pubmed":["23584611"],"doi":["10.1038/nbt.2561"]}}