{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["54(7)"],"submitter":["Acosta-Rodriguez VA"],"pubmed_abstract":["Circadian clocks regulate the temporal organization of several biochemical processes, including lipid metabolism, and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [(32)P]phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in a) the labeling of total [(3)H] GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and GPL-remodeling enzymes, such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT), respectively, with antiphase profiles. In addition, we investigate"],"journal":["Journal of lipid research"],"pagination":["1798-811"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3679383"],"repository":["biostudies-literature"],"pubmed_title":["Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions."],"pmcid":["PMC3679383"],"pubmed_authors":["Giusto NM","Salvador GA","Acosta-Rodriguez VA","Marquez S","Pasquare SJ","Gorne LD","Garbarino-Pico E","Guido ME"],"additional_accession":[]},"is_claimable":false,"name":"Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions.","description":"Circadian clocks regulate the temporal organization of several biochemical processes, including lipid metabolism, and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [(32)P]phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in a) the labeling of total [(3)H] GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and GPL-remodeling enzymes, such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT), respectively, with antiphase profiles. In addition, we investigate","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Jul","modification":"2026-05-30T07:01:42.954Z","creation":"2019-03-27T01:11:27Z"},"accession":"S-EPMC3679383","cross_references":{"pubmed":["23641021"],"doi":["10.1194/jlr.m034264","10.1194/jlr.M034264"]}}