biostudies-literatureUnknown8(6)Higareda-Almaraz JCIt has recently begun to be considered that cancer is a systemic disease and that it must be studied at every level of complexity using many of the currently available approaches, including high-throughput technologies and bioinformatics. To achieve such understanding in cervical cancer, we collected information on gene, protein and phosphoprotein expression of the HeLa cell line and performed a comprehensive analysis of the different signaling pathways, transcription networks and metabolic events in which they participate. A total expression analysis by RNA-Seq of the HeLa cell line showed that 19,974 genes were transcribed. Of these, 3,360 were over-expressed, and 2,129 under-expressed when compared to the NHEK cell line. A protein-protein interaction network was derived from the over-exPloS onee65433https://www.ebi.ac.uk/biostudies/studies/S-EPMC3680226biostudies-literatureAnalysis and prediction of pathways in HeLa cells by integrating biological levels of organization with systems-biology approaches.PMC3680226Higareda-Almaraz JCHernandez EContreras SEncarnacion-Guevara SEncarnacion SHernandez-Ortiz MValtierra-Gutierrez IAfalseAnalysis and prediction of pathways in HeLa cells by integrating biological levels of organization with systems-biology approaches.It has recently begun to be considered that cancer is a systemic disease and that it must be studied at every level of complexity using many of the currently available approaches, including high-throughput technologies and bioinformatics. To achieve such understanding in cervical cancer, we collected information on gene, protein and phosphoprotein expression of the HeLa cell line and performed a comprehensive analysis of the different signaling pathways, transcription networks and metabolic events in which they participate. A total expression analysis by RNA-Seq of the HeLa cell line showed that 19,974 genes were transcribed. Of these, 3,360 were over-expressed, and 2,129 under-expressed when compared to the NHEK cell line. A protein-protein interaction network was derived from the over-ex2013-01-01T00:00:00Z20132026-05-30T09:59:07.157Z2026-04-08T06:59:27.424ZS-EPMC36802262378542610.1371/journal.pone.0065433