biostudies-literatureYoshino TPNIAID NIH HHS463-83https://www.ebi.ac.uk/biostudies/studies/S-EPMC3682473biostudies-literatureUnknown137(3)With rapid developments in DNA and protein sequencing technologies, combined with powerful bioinformatics tools, a continued acceleration of gene identification in parasitic helminths is predicted, potentially leading to discovery of new drug and vaccine targets, enhanced diagnostics and insights into the complex biology underlying host-parasite interactions. For the schistosome blood flukes, with the recent completion of genome sequencing and comprehensive transcriptomic datasets, there has accumulated massive amounts of gene sequence data, for which, in the vast majority of cases, little is known about actual functions within the intact organism. In this review we attempt to bring together traditional in vitro cultivation approaches and recent emergent technologies of molecular genomics,ParasitologyIn vitro manipulation of gene expression in larval Schistosoma: a model for postgenomic approaches in Trematoda.PMC3682473R01 AI061436R01 AI015503R56 AI015503AI015503T32 AI007414AI061436Dinguirard NMourao Mde MYoshino TPfalseIn vitro manipulation of gene expression in larval Schistosoma: a model for postgenomic approaches in Trematoda.With rapid developments in DNA and protein sequencing technologies, combined with powerful bioinformatics tools, a continued acceleration of gene identification in parasitic helminths is predicted, potentially leading to discovery of new drug and vaccine targets, enhanced diagnostics and insights into the complex biology underlying host-parasite interactions. For the schistosome blood flukes, with the recent completion of genome sequencing and comprehensive transcriptomic datasets, there has accumulated massive amounts of gene sequence data, for which, in the vast majority of cases, little is known about actual functions within the intact organism. In this review we attempt to bring together traditional in vitro cultivation approaches and recent emergent technologies of molecular genomics,2010-01-01T00:00:00Z2010 Mar2025-06-01T12:00:52.626Z2019-03-27T01:11:37ZS-EPMC36824731996164610.1017/S003118200999130210.1017/s0031182009991302