{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li J"],"funding":["CSP VA","NIAID NIH HHS"],"pagination":["e00385-13"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3684836"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(3)"],"pubmed_abstract":["The type I interferon (IFN) signaling pathway restricts infection of many divergent families of RNA and DNA viruses by inducing hundreds of IFN-stimulated genes (ISGs), some of which have direct antiviral activity. We screened 813 short hairpin RNA (shRNA) constructs targeting 245 human ISGs using a flow cytometry approach to identify genes that modulated infection of West Nile virus (WNV) in IFN-β-treated human cells. Thirty ISGs with inhibitory effects against WNV were identified, including several novel genes that had antiviral activity against related and unrelated positive-strand RNA viruses. We also defined one ISG, activating signal cointegrator complex 3 (ASCC3), which functioned as a negative regulator of the host defense response. Silencing of ASCC3 resulted in upregulation of mu"],"journal":["mBio"],"pubmed_title":["A short hairpin RNA screen of interferon-stimulated genes identifies a novel negative regulator of the cellular antiviral response."],"pmcid":["PMC3684836"],"funding_grant_id":["HHSN272200900041C","R01 AI104972","HHSN272200900041CU19","U19 AI083019","U54 AI081680","AI083019"],"pubmed_authors":["Ding SC","Diamond MS","Gale M","Li J","Cho H","Chung BC","Chanda SK"],"additional_accession":[]},"is_claimable":false,"name":"A short hairpin RNA screen of interferon-stimulated genes identifies a novel negative regulator of the cellular antiviral response.","description":"The type I interferon (IFN) signaling pathway restricts infection of many divergent families of RNA and DNA viruses by inducing hundreds of IFN-stimulated genes (ISGs), some of which have direct antiviral activity. We screened 813 short hairpin RNA (shRNA) constructs targeting 245 human ISGs using a flow cytometry approach to identify genes that modulated infection of West Nile virus (WNV) in IFN-β-treated human cells. Thirty ISGs with inhibitory effects against WNV were identified, including several novel genes that had antiviral activity against related and unrelated positive-strand RNA viruses. We also defined one ISG, activating signal cointegrator complex 3 (ASCC3), which functioned as a negative regulator of the host defense response. Silencing of ASCC3 resulted in upregulation of mu","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Jun","modification":"2026-05-29T15:43:57.136Z","creation":"2026-05-17T03:07:40.745Z"},"accession":"S-EPMC3684836","cross_references":{"pubmed":["23781071"],"doi":["10.1128/mBio.00385-13"]}}