biostudies-literatureUnknown14Liu C<h4>Background</h4>Autophagy is a conserved cellular process that degrades and recycles cytoplasmic components via a lysosomal pathway. The phosphatidylethanolamine (PE)-conjugation of the Atg8 protein plays an important role in the yeast autophagy process. In humans, six Atg8 homologs, including MAP1LC3A, MAP1LC3B, MAP1LC3C (refer to LC3A, LC3B, and LC3C hereafter), GABARAP, GABARAPL1, and GABARAPL2 have been reported. All of them can be conjugated to PE through a ubiquitin-like conjugation system, and be located to autophagosomes.<h4>Results</h4>In this study, we found 3 new alternative splicing isoforms in LC3B, GABARAP, and GABARAPL1, (designated as LC3B-a, GABARAP-a and GABARAPL1-a, respectively). None of them can go through the PE-conjugation process and be located to autophagosomes.BMC cell biology27https://www.ebi.ac.uk/biostudies/studies/S-EPMC3686597biostudies-literatureArginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins.PMC3686597Liu JOWu JYu LMa HLiu CHuang QfalseArginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins.<h4>Background</h4>Autophagy is a conserved cellular process that degrades and recycles cytoplasmic components via a lysosomal pathway. The phosphatidylethanolamine (PE)-conjugation of the Atg8 protein plays an important role in the yeast autophagy process. In humans, six Atg8 homologs, including MAP1LC3A, MAP1LC3B, MAP1LC3C (refer to LC3A, LC3B, and LC3C hereafter), GABARAP, GABARAPL1, and GABARAPL2 have been reported. All of them can be conjugated to PE through a ubiquitin-like conjugation system, and be located to autophagosomes.<h4>Results</h4>In this study, we found 3 new alternative splicing isoforms in LC3B, GABARAP, and GABARAPL1, (designated as LC3B-a, GABARAP-a and GABARAPL1-a, respectively). None of them can go through the PE-conjugation process and be located to autophagosomes.2013-01-01T00:00:00Z2013 May2026-05-30T02:03:30.981Z2026-04-08T06:32:24.751ZS-EPMC36865972372140610.1186/1471-2121-14-27