{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(6)"],"submitter":["Kumari K"],"pubmed_abstract":["Foxtail millet (Setariaitalica L.) is a tractable experimental model crop for studying functional genomics of millets and bioenergy grasses. But the limited availability of genomic resources, particularly expressed sequence-based genic markers is significantly impeding its genetic improvement. Considering this, we attempted to develop EST-derived-SSR (eSSR) markers and utilize them in germplasm characterization, cross-genera transferability and in silico comparative mapping. From 66,027 foxtail millet EST sequences 24,828 non-redundant ESTs were deduced, representing ~16 Mb, which revealed 534 (~2%) eSSRs in 495 SSR containing ESTs at a frequency of 1/30 kb. A total of 447 pp were successfully designed, of which 327 were mapped physically onto nine chromosomes. About 106 selected primer pa"],"journal":["PloS one"],"pagination":["e67742"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3689721"],"repository":["biostudies-literature"],"pubmed_title":["Development of eSSR-Markers in Setaria italica and Their Applicability in Studying Genetic Diversity, Cross-Transferability and Comparative Mapping in Millet and Non-Millet Species."],"pmcid":["PMC3689721"],"pubmed_authors":["Gupta S","Parida SK","Muthamilarasan M","Prasad M","Chattopadhyay D","Misra G","Kumari K","Subramanian A"],"additional_accession":[]},"is_claimable":false,"name":"Development of eSSR-Markers in Setaria italica and Their Applicability in Studying Genetic Diversity, Cross-Transferability and Comparative Mapping in Millet and Non-Millet Species.","description":"Foxtail millet (Setariaitalica L.) is a tractable experimental model crop for studying functional genomics of millets and bioenergy grasses. But the limited availability of genomic resources, particularly expressed sequence-based genic markers is significantly impeding its genetic improvement. Considering this, we attempted to develop EST-derived-SSR (eSSR) markers and utilize them in germplasm characterization, cross-genera transferability and in silico comparative mapping. From 66,027 foxtail millet EST sequences 24,828 non-redundant ESTs were deduced, representing ~16 Mb, which revealed 534 (~2%) eSSRs in 495 SSR containing ESTs at a frequency of 1/30 kb. A total of 447 pp were successfully designed, of which 327 were mapped physically onto nine chromosomes. About 106 selected primer pa","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2026-05-30T12:58:43.998Z","creation":"2019-03-26T23:14:52Z"},"accession":"S-EPMC3689721","cross_references":{"pubmed":["23805325"],"doi":["10.1371/journal.pone.0067742"]}}