{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vcelakova J"],"funding":["Czech Ministry of Education"],"pagination":["589451"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3694381"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2013"],"pubmed_abstract":["Type 1 Diabetes (T1D) is considered to be a T-helper- (Th-) 1 autoimmune disease; however, T1D pathogenesis likely involves many factors, and sufficient tools for autoreactive T cell detection for the study of this disease are currently lacking. In this study, using gene expression microarrays, we analysed the effect of diabetes-associated autoantigens on peripheral blood mononuclear cells (PBMCs) with the purpose of identifying (pre)diabetes-associated cell processes. Twelve patients with recent onset T1D, 18 first-degree relatives of the TD1 patients (DRL; 9/18 autoantibody positive), and 13 healthy controls (DV) were tested. PBMCs from these individuals were stimulated with a cocktail of diabetes-associated autoantigens (proinsulin, IA-2, and GAD65-derived peptides). After 72 hours, gen"],"journal":["Journal of diabetes research"],"pubmed_title":["The effect of diabetes-associated autoantigens on cell processes in human PBMCs and their relevance to autoimmune diabetes development."],"pmcid":["PMC3694381"],"funding_grant_id":["IPL 699 001","RVO: 68378050","NPVII 2B06019","00064203"],"pubmed_authors":["Stechova K","Pithova P","Sumnik Z","Filipp D","Neuwirth A","Blatny R","Krivjanska M","Ulmannova T","Halbhuber Z","Vcelakova J","Kolar M","Kolouskova S","Petruzelkova L"],"additional_accession":[]},"is_claimable":false,"name":"The effect of diabetes-associated autoantigens on cell processes in human PBMCs and their relevance to autoimmune diabetes development.","description":"Type 1 Diabetes (T1D) is considered to be a T-helper- (Th-) 1 autoimmune disease; however, T1D pathogenesis likely involves many factors, and sufficient tools for autoreactive T cell detection for the study of this disease are currently lacking. In this study, using gene expression microarrays, we analysed the effect of diabetes-associated autoantigens on peripheral blood mononuclear cells (PBMCs) with the purpose of identifying (pre)diabetes-associated cell processes. Twelve patients with recent onset T1D, 18 first-degree relatives of the TD1 patients (DRL; 9/18 autoantibody positive), and 13 healthy controls (DV) were tested. PBMCs from these individuals were stimulated with a cocktail of diabetes-associated autoantigens (proinsulin, IA-2, and GAD65-derived peptides). After 72 hours, gen","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-06-01T12:28:30.127Z","creation":"2025-06-01T12:28:30.127Z"},"accession":"S-EPMC3694381","cross_references":{"pubmed":["23841104"],"doi":["10.1155/2013/589451"]}}