biostudies-literatureVcelakova JCzech Ministry of Education589451https://www.ebi.ac.uk/biostudies/studies/S-EPMC3694381biostudies-literatureUnknown2013Type 1 Diabetes (T1D) is considered to be a T-helper- (Th-) 1 autoimmune disease; however, T1D pathogenesis likely involves many factors, and sufficient tools for autoreactive T cell detection for the study of this disease are currently lacking. In this study, using gene expression microarrays, we analysed the effect of diabetes-associated autoantigens on peripheral blood mononuclear cells (PBMCs) with the purpose of identifying (pre)diabetes-associated cell processes. Twelve patients with recent onset T1D, 18 first-degree relatives of the TD1 patients (DRL; 9/18 autoantibody positive), and 13 healthy controls (DV) were tested. PBMCs from these individuals were stimulated with a cocktail of diabetes-associated autoantigens (proinsulin, IA-2, and GAD65-derived peptides). After 72 hours, genJournal of diabetes researchThe effect of diabetes-associated autoantigens on cell processes in human PBMCs and their relevance to autoimmune diabetes development.PMC3694381IPL 699 001RVO: 68378050NPVII 2B0601900064203Stechova KPithova PSumnik ZFilipp DNeuwirth ABlatny RKrivjanska MUlmannova THalbhuber ZVcelakova JKolar MKolouskova SPetruzelkova LfalseThe effect of diabetes-associated autoantigens on cell processes in human PBMCs and their relevance to autoimmune diabetes development.Type 1 Diabetes (T1D) is considered to be a T-helper- (Th-) 1 autoimmune disease; however, T1D pathogenesis likely involves many factors, and sufficient tools for autoreactive T cell detection for the study of this disease are currently lacking. In this study, using gene expression microarrays, we analysed the effect of diabetes-associated autoantigens on peripheral blood mononuclear cells (PBMCs) with the purpose of identifying (pre)diabetes-associated cell processes. Twelve patients with recent onset T1D, 18 first-degree relatives of the TD1 patients (DRL; 9/18 autoantibody positive), and 13 healthy controls (DV) were tested. PBMCs from these individuals were stimulated with a cocktail of diabetes-associated autoantigens (proinsulin, IA-2, and GAD65-derived peptides). After 72 hours, gen2013-01-01T00:00:00Z20132025-06-01T12:28:30.127Z2025-06-01T12:28:30.127ZS-EPMC36943812384110410.1155/2013/589451