biostudies-literatureUnknown11(1)Xie X<h4>Background</h4>Nuclear factor kappa-B (NF-κB) signalling plays an important role in diabetic nephropathy. Altered expression of connexin43 (Cx43) has been found in kidneys of diabetic animals. The aim of the current study was to investigate the role of Cx43 in the activation of NF-κB induced by high glucose in glomerular mesangial cells (GMCs) and to determine whether c-Src is involved in this process.<h4>Results</h4>We found that downregulation of Cx43 expression induced by high glucose activated NF-κB in GMCs. Orverexpression of Cx43 attenuated NF-κB p65 nuclear translocation induced by high glucose. High glucose inhibited the interaction between Cx43 and c-Src, and enhanced the interaction between c-Src and IκB-α. PP2, a c-Src inhibitor, also inhibited the tyrosine phosphorylation oCell communication and signaling : CCS38https://www.ebi.ac.uk/biostudies/studies/S-EPMC3699363biostudies-literatureConnexin43 mediates NF-κB signalling activation induced by high glucose in GMCs: involvement of c-Src.PMC3699363Lan TXie XHuang JWang SHuang HLiu PHuang KChen CShen XChang XfalseConnexin43 mediates NF-κB signalling activation induced by high glucose in GMCs: involvement of c-Src.<h4>Background</h4>Nuclear factor kappa-B (NF-κB) signalling plays an important role in diabetic nephropathy. Altered expression of connexin43 (Cx43) has been found in kidneys of diabetic animals. The aim of the current study was to investigate the role of Cx43 in the activation of NF-κB induced by high glucose in glomerular mesangial cells (GMCs) and to determine whether c-Src is involved in this process.<h4>Results</h4>We found that downregulation of Cx43 expression induced by high glucose activated NF-κB in GMCs. Orverexpression of Cx43 attenuated NF-κB p65 nuclear translocation induced by high glucose. High glucose inhibited the interaction between Cx43 and c-Src, and enhanced the interaction between c-Src and IκB-α. PP2, a c-Src inhibitor, also inhibited the tyrosine phosphorylation o2013-01-01T00:00:00Z2013 May2025-04-18T14:16:54.608Z2019-03-27T01:12:24ZS-EPMC36993632371891010.1186/1478-811X-11-38