{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Radkevich-Brown O"],"funding":["NCI NIH HHS"],"pagination":["409-17"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3702174"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["59(3)"],"pubmed_abstract":["In situ expression of a foreign antigen and an immune-modulating cytokine by intratumoral DNA electroporation was tested as a cancer therapy regimen. Transgene expression in the tumors was sustained for 2-3 weeks after intratumoral electroporation with mammalian expression plasmid containing firefly luciferase cDNA. Electroporation with cDNA encoding tetanus toxin fragment C (TetC) induced tetanus toxin-binding antibody, demonstrating immune recognition of the transgene product. Intratumoral electroporation with TetC and IL-12 cDNA after mice were treated with CD25 mAb to remove regulatory T cells induced IFN-gamma producing T-cell response to tumor-associated antigen, heavy inflammatory infiltration, regression of established tumors and immune memory to protect mice from repeated tumor challenge. Intratumoral expression of immune-modulating molecules may be most suitable in the neoadjuvant setting to enhance the therapeutic efficacy and provide long-term protection."],"journal":["Cancer immunology, immunotherapy : CII"],"pubmed_title":["Intratumoral DNA electroporation induces anti-tumor immunity  and tumor regression."],"pmcid":["PMC3702174"],"funding_grant_id":["CA125680","R01 CA076340","R01 CA125680","CA76340"],"pubmed_authors":["Pilon-Thomas S","Piechocki MP","Back JB","Weise AM","Radkevich-Brown O","Wei WZ"],"additional_accession":[]},"is_claimable":false,"name":"Intratumoral DNA electroporation induces anti-tumor immunity  and tumor regression.","description":"In situ expression of a foreign antigen and an immune-modulating cytokine by intratumoral DNA electroporation was tested as a cancer therapy regimen. Transgene expression in the tumors was sustained for 2-3 weeks after intratumoral electroporation with mammalian expression plasmid containing firefly luciferase cDNA. Electroporation with cDNA encoding tetanus toxin fragment C (TetC) induced tetanus toxin-binding antibody, demonstrating immune recognition of the transgene product. Intratumoral electroporation with TetC and IL-12 cDNA after mice were treated with CD25 mAb to remove regulatory T cells induced IFN-gamma producing T-cell response to tumor-associated antigen, heavy inflammatory infiltration, regression of established tumors and immune memory to protect mice from repeated tumor challenge. Intratumoral expression of immune-modulating molecules may be most suitable in the neoadjuvant setting to enhance the therapeutic efficacy and provide long-term protection.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Mar","modification":"2025-04-04T01:29:22.247Z","creation":"2019-03-27T01:12:33Z"},"accession":"S-EPMC3702174","cross_references":{"pubmed":["19730859"],"doi":["10.1007/s00262-009-0760-1"]}}