biostudies-literatureRadkevich-Brown ONCI NIH HHS409-17https://www.ebi.ac.uk/biostudies/studies/S-EPMC3702174biostudies-literatureUnknown59(3)In situ expression of a foreign antigen and an immune-modulating cytokine by intratumoral DNA electroporation was tested as a cancer therapy regimen. Transgene expression in the tumors was sustained for 2-3 weeks after intratumoral electroporation with mammalian expression plasmid containing firefly luciferase cDNA. Electroporation with cDNA encoding tetanus toxin fragment C (TetC) induced tetanus toxin-binding antibody, demonstrating immune recognition of the transgene product. Intratumoral electroporation with TetC and IL-12 cDNA after mice were treated with CD25 mAb to remove regulatory T cells induced IFN-gamma producing T-cell response to tumor-associated antigen, heavy inflammatory infiltration, regression of established tumors and immune memory to protect mice from repeated tumor challenge. Intratumoral expression of immune-modulating molecules may be most suitable in the neoadjuvant setting to enhance the therapeutic efficacy and provide long-term protection.Cancer immunology, immunotherapy : CIIIntratumoral DNA electroporation induces anti-tumor immunity and tumor regression.PMC3702174CA125680R01 CA076340R01 CA125680CA76340Pilon-Thomas SPiechocki MPBack JBWeise AMRadkevich-Brown OWei WZfalseIntratumoral DNA electroporation induces anti-tumor immunity and tumor regression.In situ expression of a foreign antigen and an immune-modulating cytokine by intratumoral DNA electroporation was tested as a cancer therapy regimen. Transgene expression in the tumors was sustained for 2-3 weeks after intratumoral electroporation with mammalian expression plasmid containing firefly luciferase cDNA. Electroporation with cDNA encoding tetanus toxin fragment C (TetC) induced tetanus toxin-binding antibody, demonstrating immune recognition of the transgene product. Intratumoral electroporation with TetC and IL-12 cDNA after mice were treated with CD25 mAb to remove regulatory T cells induced IFN-gamma producing T-cell response to tumor-associated antigen, heavy inflammatory infiltration, regression of established tumors and immune memory to protect mice from repeated tumor challenge. Intratumoral expression of immune-modulating molecules may be most suitable in the neoadjuvant setting to enhance the therapeutic efficacy and provide long-term protection.2010-01-01T00:00:00Z2010 Mar2025-04-04T01:29:22.247Z2019-03-27T01:12:33ZS-EPMC37021741973085910.1007/s00262-009-0760-1