{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Santhanam L"],"funding":["NIA NIH HHS","NIDDK NIH HHS","NHLBI NIH HHS","NCI NIH HHS"],"pagination":["295-302"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3705760"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(3)"],"pubmed_abstract":["Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that eliminates the need for Western blot or avidin enrichment protocols and allows direct comparison of the S-nitrosation state proteins from two different samples in the same gel lane or on the same 2D gel. This S-FLOS method offers detection, identification and quantification of S-nitrosated proteins, with the potential for site-specific identification of nitrosation events."],"journal":["Nitric oxide : biology and chemistry"],"pubmed_title":["Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation."],"pmcid":["PMC3705760"],"funding_grant_id":["R24 DK064388","P30 CA006973","R01 AG021523","N01-HV-28180","N01 HV028180","N01HV28180"],"pubmed_authors":["Mansharamani M","Ryoo S","Romer L","Berkowitz DE","Shoukas AA","Brown TR","Santhanam L","Lemmon CA","Gucek M","Cole RN"],"additional_accession":[]},"is_claimable":false,"name":"Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation.","description":"Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that eliminates the need for Western blot or avidin enrichment protocols and allows direct comparison of the S-nitrosation state proteins from two different samples in the same gel lane or on the same 2D gel. This S-FLOS method offers detection, identification and quantification of S-nitrosated proteins, with the potential for site-specific identification of nitrosation events.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Nov","modification":"2025-04-26T20:29:59.03Z","creation":"2019-03-27T01:12:42Z"},"accession":"S-EPMC3705760","cross_references":{"pubmed":["18706513"],"doi":["10.1016/j.niox.2008.07.007"]}}