biostudies-literatureKelly ADNCI NIH HHS2https://www.ebi.ac.uk/biostudies/studies/S-EPMC3706900biostudies-literatureUnknown5(1)<h4>Background</h4>Although microRNAs (miRNAs) are implicated in osteosarcoma biology and chemoresponse, miRNA prognostic models are still needed, particularly because prognosis is imperfectly correlated with chemoresponse. Formalin-fixed, paraffin-embedded tissue is a necessary resource for biomarker studies in this malignancy with limited frozen tissue availability.<h4>Methods</h4>We performed miRNA and mRNA microarray formalin-fixed, paraffin-embedded assays in 65 osteosarcoma biopsy and 26 paired post-chemotherapy resection specimens and used the only publicly available miRNA dataset, generated independently by another group, to externally validate our strongest findings (n = 29). We used supervised principal components analysis and logistic regression for survival and chemoresponse, aGenome medicineMicroRNA paraffin-based studies in osteosarcoma reveal reproducible independent prognostic profiles at 14q32.PMC3706900K22 CA138716April CKelly ADHill KEGoldsmith JKurek KHowe EJaneway KAFrancoeur NSchneider HQuackenbush JSpentzos DFan JBHaibe-Kains BGebhardt MCCulhane APerez-Atayde ARfalseMicroRNA paraffin-based studies in osteosarcoma reveal reproducible independent prognostic profiles at 14q32.<h4>Background</h4>Although microRNAs (miRNAs) are implicated in osteosarcoma biology and chemoresponse, miRNA prognostic models are still needed, particularly because prognosis is imperfectly correlated with chemoresponse. Formalin-fixed, paraffin-embedded tissue is a necessary resource for biomarker studies in this malignancy with limited frozen tissue availability.<h4>Methods</h4>We performed miRNA and mRNA microarray formalin-fixed, paraffin-embedded assays in 65 osteosarcoma biopsy and 26 paired post-chemotherapy resection specimens and used the only publicly available miRNA dataset, generated independently by another group, to externally validate our strongest findings (n = 29). We used supervised principal components analysis and logistic regression for survival and chemoresponse, a2013-01-01T00:00:00Z20132025-04-26T20:28:09.207Z2019-03-27T01:12:46ZS-EPMC37069002333946210.1186/gm406