biostudies-literatureKiaii SNational Institute for Health Research (NIHR)NCI NIH HHS2654-61https://www.ebi.ac.uk/biostudies/studies/S-EPMC3709054biostudies-literatureUnknown31(21)<h4>Purpose</h4>Previous studies have demonstrated the prognostic importance of the immune microenvironment in follicular lymphoma (FL). To investigate the molecular mechanisms during which tumor-infiltrating T cells (TILs) are altered in the FL microenvironment, we studied highly purified CD4 and CD8 TILs from lymph node biopsies at diagnosis in treatment-naive patients with FL compared with reactive tonsils and the peripheral blood of healthy donors.<h4>Patients and methods</h4>Gene expression profiling of highly purified CD4 and CD8 TILs was performed on the Affymetrix platform. Diagnostic tissue microarrays from an independent patient set (n = 172) were used to verify protein expression and analyze any impact of TIL-expressed genes on outcome. Time-lapse imaging was used to assess T-ceJournal of clinical oncology : official journal of the American Society of Clinical OncologyFollicular lymphoma cells induce changes in T-cell gene expression and function: potential impact on survival and risk of transformation.PMC3709054P01 CA081534CL-2007-19-002Neuberg DSLee ASangaralingam ACalaminici MGribben JGClear AJRamsay AGDavies DKiaii SfalseFollicular lymphoma cells induce changes in T-cell gene expression and function: potential impact on survival and risk of transformation.<h4>Purpose</h4>Previous studies have demonstrated the prognostic importance of the immune microenvironment in follicular lymphoma (FL). To investigate the molecular mechanisms during which tumor-infiltrating T cells (TILs) are altered in the FL microenvironment, we studied highly purified CD4 and CD8 TILs from lymph node biopsies at diagnosis in treatment-naive patients with FL compared with reactive tonsils and the peripheral blood of healthy donors.<h4>Patients and methods</h4>Gene expression profiling of highly purified CD4 and CD8 TILs was performed on the Affymetrix platform. Diagnostic tissue microarrays from an independent patient set (n = 172) were used to verify protein expression and analyze any impact of TIL-expressed genes on outcome. Time-lapse imaging was used to assess T-ce2013-01-01T00:00:00Z2013 Jul2026-06-04T17:16:19.675Z2019-03-26T22:59:43ZS-EPMC37090542377595910.1200/JCO.2012.44.213710.1200/jco.2012.44.2137