{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(6)"],"submitter":["Thomas M"],"pubmed_abstract":["The human polycistronic miRNA cluster miR-17-92 is frequently overexpressed in hematopoietic malignancies and cancers. Its transcription is in part controlled by an E2F-regulated host gene promoter. An intronic A/T-rich region directly upstream of the miRNA coding region also contributes to cluster expression. Our deletion analysis of the A/T-rich region revealed a strong dependence on c-Myc binding to the functional E3 site. Yet, constructs lacking the 5'-proximal ~1.3 kb or 3'-distal ~0.1 kb of the 1.5 kb A/T-rich region still retained residual specific promoter activity, suggesting multiple transcription start sites (TSS) in this region. Furthermore, the protooncogenic kinase, Pim-1, its phosphorylation target HP1γ and c-Myc colocalize to the E3 region, as inferred from chromatin immuno"],"journal":["International journal of molecular sciences"],"pagination":["12273-96"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3709785"],"repository":["biostudies-literature"],"pubmed_title":["Analysis of transcriptional regulation of the human miR-17-92 cluster; evidence for involvement of Pim-1."],"pmcid":["PMC3709785"],"pubmed_authors":["Thomas M","Grunweller A","Golde L","Aigner A","Hartmann D","Lange-Grunweller K","Schlereth J","Streng D","Hartmann RK"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of transcriptional regulation of the human miR-17-92 cluster; evidence for involvement of Pim-1.","description":"The human polycistronic miRNA cluster miR-17-92 is frequently overexpressed in hematopoietic malignancies and cancers. Its transcription is in part controlled by an E2F-regulated host gene promoter. An intronic A/T-rich region directly upstream of the miRNA coding region also contributes to cluster expression. Our deletion analysis of the A/T-rich region revealed a strong dependence on c-Myc binding to the functional E3 site. Yet, constructs lacking the 5'-proximal ~1.3 kb or 3'-distal ~0.1 kb of the 1.5 kb A/T-rich region still retained residual specific promoter activity, suggesting multiple transcription start sites (TSS) in this region. Furthermore, the protooncogenic kinase, Pim-1, its phosphorylation target HP1γ and c-Myc colocalize to the E3 region, as inferred from chromatin immuno","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Jun","modification":"2026-04-08T17:03:31.495Z","creation":"2019-03-27T01:12:56Z"},"accession":"S-EPMC3709785","cross_references":{"pubmed":["23749113"],"doi":["10.3390/ijms140612273"]}}