biostudies-literatureUnknown14(6)Thomas MThe human polycistronic miRNA cluster miR-17-92 is frequently overexpressed in hematopoietic malignancies and cancers. Its transcription is in part controlled by an E2F-regulated host gene promoter. An intronic A/T-rich region directly upstream of the miRNA coding region also contributes to cluster expression. Our deletion analysis of the A/T-rich region revealed a strong dependence on c-Myc binding to the functional E3 site. Yet, constructs lacking the 5'-proximal ~1.3 kb or 3'-distal ~0.1 kb of the 1.5 kb A/T-rich region still retained residual specific promoter activity, suggesting multiple transcription start sites (TSS) in this region. Furthermore, the protooncogenic kinase, Pim-1, its phosphorylation target HP1γ and c-Myc colocalize to the E3 region, as inferred from chromatin immunoInternational journal of molecular sciences12273-96https://www.ebi.ac.uk/biostudies/studies/S-EPMC3709785biostudies-literatureAnalysis of transcriptional regulation of the human miR-17-92 cluster; evidence for involvement of Pim-1.PMC3709785Thomas MGrunweller AGolde LAigner AHartmann DLange-Grunweller KSchlereth JStreng DHartmann RKfalseAnalysis of transcriptional regulation of the human miR-17-92 cluster; evidence for involvement of Pim-1.The human polycistronic miRNA cluster miR-17-92 is frequently overexpressed in hematopoietic malignancies and cancers. Its transcription is in part controlled by an E2F-regulated host gene promoter. An intronic A/T-rich region directly upstream of the miRNA coding region also contributes to cluster expression. Our deletion analysis of the A/T-rich region revealed a strong dependence on c-Myc binding to the functional E3 site. Yet, constructs lacking the 5'-proximal ~1.3 kb or 3'-distal ~0.1 kb of the 1.5 kb A/T-rich region still retained residual specific promoter activity, suggesting multiple transcription start sites (TSS) in this region. Furthermore, the protooncogenic kinase, Pim-1, its phosphorylation target HP1γ and c-Myc colocalize to the E3 region, as inferred from chromatin immuno2013-01-01T00:00:00Z2013 Jun2026-04-08T17:03:31.495Z2019-03-27T01:12:56ZS-EPMC37097852374911310.3390/ijms140612273