biostudies-literatureUnknown8(7)Olaya-Abril APurified polysaccharide and conjugate vaccines are widely used for preventing infections in adults and in children against the Gram-positive bacterium Streptococcus pneumoniae, a pathogen responsible for high morbidity and mortality rates, especially in developing countries. However, these polysaccharide-based vaccines have some important limitations, such as being serotype-dependent, being subjected to losing efficacy because of serotype replacement and high manufacturing complexity and cost. It is expected that protein-based vaccines will overcome these issues by conferring a broad coverage independent of serotype and lowering production costs. In this study, we have applied the "shaving" proteomic approach, consisting of the LC/MS/MS analysis of peptides generated by protease treatment PloS onee70365https://www.ebi.ac.uk/biostudies/studies/S-EPMC3720901biostudies-literatureIdentification of potential new protein vaccine candidates through pan-surfomic analysis of pneumococcal clinical isolates from adults.PMC3720901Jimenez-Munguia IGomez-Gascon LObando IRodriguez-Ortega MJOlaya-Abril AfalseIdentification of potential new protein vaccine candidates through pan-surfomic analysis of pneumococcal clinical isolates from adults.Purified polysaccharide and conjugate vaccines are widely used for preventing infections in adults and in children against the Gram-positive bacterium Streptococcus pneumoniae, a pathogen responsible for high morbidity and mortality rates, especially in developing countries. However, these polysaccharide-based vaccines have some important limitations, such as being serotype-dependent, being subjected to losing efficacy because of serotype replacement and high manufacturing complexity and cost. It is expected that protein-based vaccines will overcome these issues by conferring a broad coverage independent of serotype and lowering production costs. In this study, we have applied the "shaving" proteomic approach, consisting of the LC/MS/MS analysis of peptides generated by protease treatment 2013-01-01T00:00:00Z20132025-04-26T20:26:05.228Z2019-03-26T23:15:12ZS-EPMC37209012389464110.1371/journal.pone.0070365