{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Padi SK"],"funding":["NCRR NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["437-46"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3722307"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["145(2)"],"pubmed_abstract":["<h4>Background & aims</h4>Vitamin D protects against colorectal cancer through unclear mechanisms. We investigated the effects of calcitriol (1α,25-dihydroxyvitamin D3; the active form of vitamin D) on levels of different microRNAs (miRNAs) in colorectal cancer cells from humans and xenograft tumors in mice.<h4>Methods</h4>Expression of miRNAs in colorectal cancer cell lines was examined using the Ambion mirVana miRNA Bioarray. The effects of calcitriol on expression of miR-627 and cell proliferation were determined by real-time polymerase chain reaction and WST-1 assay, respectively; growth of colorectal xenograft tumors was examined in nude mice. Real-time polymerase chain reaction was used to analyze levels of miR-627 in human colon adenocarcinoma samples and nontumor colon mucosa tissu"],"journal":["Gastroenterology"],"pubmed_title":["MicroRNA-627 mediates the epigenetic mechanisms of vitamin D to suppress proliferation of human colorectal cancer cells and growth of xenograft tumors in mice."],"pmcid":["PMC3722307"],"funding_grant_id":["RR015566","P30 CA016056","P30 GM103332","P20 RR015566","R03 CA130062","CA130062"],"pubmed_authors":["Padi SK","Morrison C","Guo B","Zhang Q","Rustum YM"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-627 mediates the epigenetic mechanisms of vitamin D to suppress proliferation of human colorectal cancer cells and growth of xenograft tumors in mice.","description":"<h4>Background & aims</h4>Vitamin D protects against colorectal cancer through unclear mechanisms. We investigated the effects of calcitriol (1α,25-dihydroxyvitamin D3; the active form of vitamin D) on levels of different microRNAs (miRNAs) in colorectal cancer cells from humans and xenograft tumors in mice.<h4>Methods</h4>Expression of miRNAs in colorectal cancer cell lines was examined using the Ambion mirVana miRNA Bioarray. The effects of calcitriol on expression of miR-627 and cell proliferation were determined by real-time polymerase chain reaction and WST-1 assay, respectively; growth of colorectal xenograft tumors was examined in nude mice. Real-time polymerase chain reaction was used to analyze levels of miR-627 in human colon adenocarcinoma samples and nontumor colon mucosa tissu","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Aug","modification":"2025-04-03T23:50:41.414Z","creation":"2019-03-26T23:05:25Z"},"accession":"S-EPMC3722307","cross_references":{"pubmed":["23619147"],"doi":["10.1053/j.gastro.2013.04.012"]}}