biostudies-literaturePadi SKNCRR NIH HHSNCI NIH HHSNIGMS NIH HHS437-46https://www.ebi.ac.uk/biostudies/studies/S-EPMC3722307biostudies-literatureUnknown145(2)<h4>Background & aims</h4>Vitamin D protects against colorectal cancer through unclear mechanisms. We investigated the effects of calcitriol (1α,25-dihydroxyvitamin D3; the active form of vitamin D) on levels of different microRNAs (miRNAs) in colorectal cancer cells from humans and xenograft tumors in mice.<h4>Methods</h4>Expression of miRNAs in colorectal cancer cell lines was examined using the Ambion mirVana miRNA Bioarray. The effects of calcitriol on expression of miR-627 and cell proliferation were determined by real-time polymerase chain reaction and WST-1 assay, respectively; growth of colorectal xenograft tumors was examined in nude mice. Real-time polymerase chain reaction was used to analyze levels of miR-627 in human colon adenocarcinoma samples and nontumor colon mucosa tissuGastroenterologyMicroRNA-627 mediates the epigenetic mechanisms of vitamin D to suppress proliferation of human colorectal cancer cells and growth of xenograft tumors in mice.PMC3722307RR015566P30 CA016056P30 GM103332P20 RR015566R03 CA130062CA130062Padi SKMorrison CGuo BZhang QRustum YMfalseMicroRNA-627 mediates the epigenetic mechanisms of vitamin D to suppress proliferation of human colorectal cancer cells and growth of xenograft tumors in mice.<h4>Background & aims</h4>Vitamin D protects against colorectal cancer through unclear mechanisms. We investigated the effects of calcitriol (1α,25-dihydroxyvitamin D3; the active form of vitamin D) on levels of different microRNAs (miRNAs) in colorectal cancer cells from humans and xenograft tumors in mice.<h4>Methods</h4>Expression of miRNAs in colorectal cancer cell lines was examined using the Ambion mirVana miRNA Bioarray. The effects of calcitriol on expression of miR-627 and cell proliferation were determined by real-time polymerase chain reaction and WST-1 assay, respectively; growth of colorectal xenograft tumors was examined in nude mice. Real-time polymerase chain reaction was used to analyze levels of miR-627 in human colon adenocarcinoma samples and nontumor colon mucosa tissu2013-01-01T00:00:00Z2013 Aug2025-04-03T23:50:41.414Z2019-03-26T23:05:25ZS-EPMC37223072361914710.1053/j.gastro.2013.04.012