biostudies-literatureMinvielle MJNIGMS NIH HHS916-919https://www.ebi.ac.uk/biostudies/studies/S-EPMC3733276biostudies-literatureUnknown4(6)Herein is described a method of accessing indole/triazole and benzothiophene/triazole analogues that selectively promote or inhibit biofilm formation by Gram-positive and Gram-negative bacteria. Structure/function studies revealed that the addition of a bromine atom at the 2-position of the indole/triazole scaffold altered activity against both Gram-negative and Gram-positive bacteria and could transform a biofilm inhibitor into a biofilm inducer. Isosteric replacement of the indole core by a benzothiophene significantly impaired anti-biofilm activity. A competition assay exposing <i>Escherichia coli</i> to the most potent biofilm inducer and an inhibitor of <i>E. coli</i> biofilm formation was performed. The inducer exhibited the ability to mute the effect of the anti-biofilm compound for this targeted bacterial population.MedChemCommIndole/triazole conjugates are selective inhibitors and inducers of bacterial biofilms.PMC3733276R01 GM055769Minvielle MJBunders CAMelander CfalseIndole/triazole conjugates are selective inhibitors and inducers of bacterial biofilms.Herein is described a method of accessing indole/triazole and benzothiophene/triazole analogues that selectively promote or inhibit biofilm formation by Gram-positive and Gram-negative bacteria. Structure/function studies revealed that the addition of a bromine atom at the 2-position of the indole/triazole scaffold altered activity against both Gram-negative and Gram-positive bacteria and could transform a biofilm inhibitor into a biofilm inducer. Isosteric replacement of the indole core by a benzothiophene significantly impaired anti-biofilm activity. A competition assay exposing <i>Escherichia coli</i> to the most potent biofilm inducer and an inhibitor of <i>E. coli</i> biofilm formation was performed. The inducer exhibited the ability to mute the effect of the anti-biofilm compound for this targeted bacterial population.2013-01-01T00:00:00Z2013 Jun2025-04-07T13:01:30.368Z2019-03-26T23:51:07ZS-EPMC37332762393019910.1039/C3MD00064H10.1039/c3md00064h