{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3(1)"],"submitter":["Tomisawa S"],"pubmed_abstract":["Antibacterial factor 2 (ABF-2) is a 67-residue antimicrobial peptide derived from the nematode Caenorhabditis elegans. Although it has been reported that ABF-2 exerts in vitro microbicidal activity against a range of bacteria and fungi, the structure of ABF-2 has not yet been solved. To enable structural studies of ABF-2 by NMR spectroscopy, a large amount of isotopically labeled ABF-2 is essential. However, the direct expression of ABF-2 in Escherichia coli is difficult to achieve due to its instability. Therefore, we applied a coexpression method to the production of ABF-2 in order to enhance the inclusion body formation of ABF-2. The inclusion body formation of ABF-2 was vastly enhanced by coexpression of aggregation-prone proteins (partner proteins). By using this method, we succeeded "],"journal":["AMB Express"],"pagination":["45"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3751704"],"repository":["biostudies-literature"],"pubmed_title":["Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system."],"pmcid":["PMC3751704"],"pubmed_authors":["Kato Y","Kawano K","Kikukawa T","Demura M","Kumaki Y","Ohki S","Mizuguchi M","Miyazawa M","Kamiya M","Aizawa T","Hojo E","Tomisawa S","Umetsu Y"],"additional_accession":[]},"is_claimable":false,"name":"Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system.","description":"Antibacterial factor 2 (ABF-2) is a 67-residue antimicrobial peptide derived from the nematode Caenorhabditis elegans. Although it has been reported that ABF-2 exerts in vitro microbicidal activity against a range of bacteria and fungi, the structure of ABF-2 has not yet been solved. To enable structural studies of ABF-2 by NMR spectroscopy, a large amount of isotopically labeled ABF-2 is essential. However, the direct expression of ABF-2 in Escherichia coli is difficult to achieve due to its instability. Therefore, we applied a coexpression method to the production of ABF-2 in order to enhance the inclusion body formation of ABF-2. The inclusion body formation of ABF-2 was vastly enhanced by coexpression of aggregation-prone proteins (partner proteins). By using this method, we succeeded ","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Aug","modification":"2025-04-18T15:10:39.37Z","creation":"2019-03-27T01:14:58Z"},"accession":"S-EPMC3751704","cross_references":{"pubmed":["23945047"],"doi":["10.1186/2191-0855-3-45"]}}