biostudies-literatureUnknown3(1)Tomisawa SAntibacterial factor 2 (ABF-2) is a 67-residue antimicrobial peptide derived from the nematode Caenorhabditis elegans. Although it has been reported that ABF-2 exerts in vitro microbicidal activity against a range of bacteria and fungi, the structure of ABF-2 has not yet been solved. To enable structural studies of ABF-2 by NMR spectroscopy, a large amount of isotopically labeled ABF-2 is essential. However, the direct expression of ABF-2 in Escherichia coli is difficult to achieve due to its instability. Therefore, we applied a coexpression method to the production of ABF-2 in order to enhance the inclusion body formation of ABF-2. The inclusion body formation of ABF-2 was vastly enhanced by coexpression of aggregation-prone proteins (partner proteins). By using this method, we succeeded AMB Express45https://www.ebi.ac.uk/biostudies/studies/S-EPMC3751704biostudies-literatureOverexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system.PMC3751704Kato YKawano KKikukawa TDemura MKumaki YOhki SMizuguchi MMiyazawa MKamiya MAizawa THojo ETomisawa SUmetsu YfalseOverexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system.Antibacterial factor 2 (ABF-2) is a 67-residue antimicrobial peptide derived from the nematode Caenorhabditis elegans. Although it has been reported that ABF-2 exerts in vitro microbicidal activity against a range of bacteria and fungi, the structure of ABF-2 has not yet been solved. To enable structural studies of ABF-2 by NMR spectroscopy, a large amount of isotopically labeled ABF-2 is essential. However, the direct expression of ABF-2 in Escherichia coli is difficult to achieve due to its instability. Therefore, we applied a coexpression method to the production of ABF-2 in order to enhance the inclusion body formation of ABF-2. The inclusion body formation of ABF-2 was vastly enhanced by coexpression of aggregation-prone proteins (partner proteins). By using this method, we succeeded 2013-01-01T00:00:00Z2013 Aug2025-04-18T15:10:39.37Z2019-03-27T01:14:58ZS-EPMC37517042394504710.1186/2191-0855-3-45