{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee BS"],"funding":["NIAID NIH HHS","NCI NIH HHS","NIGMS NIH HHS","CIHR"],"pagination":["3568-79"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3753869"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(18)"],"pubmed_abstract":["V(D)J recombination is initiated by the RAG endonuclease, which introduces DNA double-strand breaks (DSBs) at the border between two recombining gene segments, generating two hairpin-sealed coding ends and two blunt signal ends. ATM and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) are serine-threonine kinases that orchestrate the cellular responses to DNA DSBs. During V(D)J recombination, ATM and DNA-PKcs have unique functions in the repair of coding DNA ends. ATM deficiency leads to instability of postcleavage complexes and the loss of coding ends from these complexes. DNA-PKcs deficiency leads to a nearly complete block in coding join formation, as DNA-PKcs is required to activate Artemis, the endonuclease that opens hairpin-sealed coding ends. In contrast to loss of DNA-PKc"],"journal":["Molecular and cellular biology"],"pubmed_title":["Functional intersection of ATM and DNA-dependent protein kinase catalytic subunit in coding end joining during V(D)J recombination."],"pmcid":["PMC3753869"],"funding_grant_id":["GM105404","R01 AI047829","R01 GM105404","691369","R01 CA166677","AI47829","AI074953","R01 CA136470","P01 CA092584","CA92584","CA136470","R01 AI074953"],"pubmed_authors":["Osipovich O","Daniel JA","Lee BS","Dorsett Y","Zhang S","Gapud EJ","Hammel M","Bredemeyer A","Sleckman BP","Lees-Miller S","Callen E","Nussenzweig A","Oltz EM","George R","Bassing CH","Chen BP"],"additional_accession":[]},"is_claimable":false,"name":"Functional intersection of ATM and DNA-dependent protein kinase catalytic subunit in coding end joining during V(D)J recombination.","description":"V(D)J recombination is initiated by the RAG endonuclease, which introduces DNA double-strand breaks (DSBs) at the border between two recombining gene segments, generating two hairpin-sealed coding ends and two blunt signal ends. ATM and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) are serine-threonine kinases that orchestrate the cellular responses to DNA DSBs. During V(D)J recombination, ATM and DNA-PKcs have unique functions in the repair of coding DNA ends. ATM deficiency leads to instability of postcleavage complexes and the loss of coding ends from these complexes. DNA-PKcs deficiency leads to a nearly complete block in coding join formation, as DNA-PKcs is required to activate Artemis, the endonuclease that opens hairpin-sealed coding ends. In contrast to loss of DNA-PKc","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Sep","modification":"2026-05-06T21:38:35.784Z","creation":"2026-04-07T22:21:53.406Z"},"accession":"S-EPMC3753869","cross_references":{"pubmed":["23836881"],"doi":["10.1128/mcb.00308-13","10.1128/MCB.00308-13"]}}