biostudies-literatureMaglennon GABiotechnology and Biological Sciences Research Council63https://www.ebi.ac.uk/biostudies/studies/S-EPMC3765554biostudies-literatureUnknown44Mycoplasma hyopneumoniae is a prevalent swine respiratory pathogen that is a major cause of economic loss to pig producers. Control is achieved by a combination of antimicrobials, vaccination and management practices, but current vaccines offer only partial control and there is a need for improved preventative strategies. A major barrier to advances in understanding the pathogenesis of M. hyopneumoniae and in developing new vaccines is the lack of tools to genetically manipulate the organism. We describe the development and optimisation of the first successful plasmid-based system for the genetic manipulation of M. hyopneumoniae. Our artificial plasmids contain the origin of replication (oriC) of M. hyopneumoniae along with tetM, conferring resistance to tetracycline. With these plasmids, Veterinary researchDevelopment of a self-replicating plasmid system for Mycoplasma hyopneumoniae.PMC3765554BB/G019274/1BB/G018553/1BB/G019177/1BB/G020744/1Tucker AWWeinert LDeeney ASMaglennon GATerra VLuan SLLangford PRMatthews DMaskell DJPeters SCook BSCuccui JLi YBosse JTRycroft ANChaudhuri RWang TWren BWBRaDP1T consortiumfalseDevelopment of a self-replicating plasmid system for Mycoplasma hyopneumoniae.Mycoplasma hyopneumoniae is a prevalent swine respiratory pathogen that is a major cause of economic loss to pig producers. Control is achieved by a combination of antimicrobials, vaccination and management practices, but current vaccines offer only partial control and there is a need for improved preventative strategies. A major barrier to advances in understanding the pathogenesis of M. hyopneumoniae and in developing new vaccines is the lack of tools to genetically manipulate the organism. We describe the development and optimisation of the first successful plasmid-based system for the genetic manipulation of M. hyopneumoniae. Our artificial plasmids contain the origin of replication (oriC) of M. hyopneumoniae along with tetM, conferring resistance to tetracycline. With these plasmids, 2013-01-01T00:00:00Z2013 Jul2026-05-30T09:52:42.203Z2019-03-27T01:15:39ZS-EPMC37655542389523610.1186/1297-9716-44-63