{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(9)"],"submitter":["Lugovtsev VY"],"pubmed_abstract":["Single-cell clones have been established from the MDCK cell line, characterized for their morphology and evaluated for their suitability for influenza virus research. Three discrete cell morphotypes were identified using light microscopy. Besides morphological features, the cell types can be distinguished by the level of expression of surface glycans recognized by peanut agglutinin (PNA). All clones were susceptible to infection by influenza viruses of different subtypes of influenza A virus (H1N1, H1N1pdm09, H3N2, H5N1) and influenza B virus, and all possessed on their surface terminally sialylated glycans with both types of glycosidic linkage (α2-3 and α2-6). The Type-1 cell lines were able to support a multicycle replication of influenza A and B viruses without help of an exogenous tryp"],"journal":["PloS one"],"pagination":["e75014"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3772841"],"repository":["biostudies-literature"],"pubmed_title":["Heterogeneity of the MDCK cell line and its applicability for influenza virus research."],"pmcid":["PMC3772841"],"pubmed_authors":["Melnyk D","Lugovtsev VY","Weir JP"],"additional_accession":[]},"is_claimable":false,"name":"Heterogeneity of the MDCK cell line and its applicability for influenza virus research.","description":"Single-cell clones have been established from the MDCK cell line, characterized for their morphology and evaluated for their suitability for influenza virus research. Three discrete cell morphotypes were identified using light microscopy. Besides morphological features, the cell types can be distinguished by the level of expression of surface glycans recognized by peanut agglutinin (PNA). All clones were susceptible to infection by influenza viruses of different subtypes of influenza A virus (H1N1, H1N1pdm09, H3N2, H5N1) and influenza B virus, and all possessed on their surface terminally sialylated glycans with both types of glycosidic linkage (α2-3 and α2-6). The Type-1 cell lines were able to support a multicycle replication of influenza A and B viruses without help of an exogenous tryp","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-21T15:50:23.8Z","creation":"2019-03-26T23:21:07Z"},"accession":"S-EPMC3772841","cross_references":{"pubmed":["24058646"],"doi":["10.1371/journal.pone.0075014"]}}