{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mierzejewska K"],"funding":["NIDDK NIH HHS","NCRR NIH HHS"],"pagination":["281-90"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3776718"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["32(2)"],"pubmed_abstract":["It has been postulated that the most primitive population of stem cells, Oct4(+)Sca-1(+)Lin(-)CD45(-) very small embryonic-like stem cells (VSELs), differentiate into tissue-committed stem cells in adult mice. However, Oct4(+) VSELs remain quiescent in adult tissues and do not form teratomas. In thi study, we report the characteristics of the VSEL transcriptome by gene set enrichment analysis employing a microarray database established from 20 murine bone marrow-derived, FACS-sorted VSELs in comparison with hematopoietic stem cells and embryonic stem cells. In the Oct4(+) VSELs, we observed the upregulation of tissue-specific gene sets and a gene set encoding the complement-coagulation cascade. By contrast, in the VSELs, we observed the downregulation of genes involved in the UV radiation "],"journal":["International journal of molecular medicine"],"pubmed_title":["Genome-wide analysis of murine bone marrow‑derived very small embryonic-like stem cells reveals that mitogenic growth factor signaling pathways play a crucial role in the quiescence and ageing of these cells."],"pmcid":["PMC3776718"],"funding_grant_id":["P20 RR018733","P20RR018733","R01 DK074720"],"pubmed_authors":["Kucia M","Kang H","Mierzejewska K","Kang JW","Shin DM","Heo J","Ratajczak J","Ratajczak MZ"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide analysis of murine bone marrow‑derived very small embryonic-like stem cells reveals that mitogenic growth factor signaling pathways play a crucial role in the quiescence and ageing of these cells.","description":"It has been postulated that the most primitive population of stem cells, Oct4(+)Sca-1(+)Lin(-)CD45(-) very small embryonic-like stem cells (VSELs), differentiate into tissue-committed stem cells in adult mice. However, Oct4(+) VSELs remain quiescent in adult tissues and do not form teratomas. In thi study, we report the characteristics of the VSEL transcriptome by gene set enrichment analysis employing a microarray database established from 20 murine bone marrow-derived, FACS-sorted VSELs in comparison with hematopoietic stem cells and embryonic stem cells. In the Oct4(+) VSELs, we observed the upregulation of tissue-specific gene sets and a gene set encoding the complement-coagulation cascade. By contrast, in the VSELs, we observed the downregulation of genes involved in the UV radiation ","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Aug","modification":"2025-04-18T19:50:46.701Z","creation":"2019-06-05T19:18:05Z"},"accession":"S-EPMC3776718","cross_references":{"pubmed":["23708325"],"doi":["10.3892/ijmm.2013.1389"]}}