{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ni TK"],"funding":["Howard Hughes Medical Institute","NHGRI NIH HHS","NIGMS NIH HHS"],"pagination":["E3640-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3780872"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["110(38)"],"pubmed_abstract":["Despite considerable efforts to sequence hypermutated cancers such as melanoma, distinguishing cancer-driving genes from thousands of recurrently mutated genes remains a significant challenge. To circumvent the problematic background mutation rates and identify new melanoma driver genes, we carried out a low-copy piggyBac transposon mutagenesis screen in mice. We induced eleven melanomas with mutation burdens that were 100-fold lower relative to human melanomas. Thirty-eight implicated genes, including two known drivers of human melanoma, were classified into three groups based on high, low, or background-level mutation frequencies in human melanomas, and we further explored the functional significance of genes in each group. For two genes overlooked by prevailing discovery methods, we fou"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Low-copy piggyBac transposon mutagenesis in mice identifies genes driving melanoma."],"pmcid":["PMC3780872"],"funding_grant_id":["T32 GM007499","T32 HG003198"],"pubmed_authors":["Landrette SF","Bosenberg MW","Ni TK","Xu T","Bjornson RD"],"additional_accession":[]},"is_claimable":false,"name":"Low-copy piggyBac transposon mutagenesis in mice identifies genes driving melanoma.","description":"Despite considerable efforts to sequence hypermutated cancers such as melanoma, distinguishing cancer-driving genes from thousands of recurrently mutated genes remains a significant challenge. To circumvent the problematic background mutation rates and identify new melanoma driver genes, we carried out a low-copy piggyBac transposon mutagenesis screen in mice. We induced eleven melanomas with mutation burdens that were 100-fold lower relative to human melanomas. Thirty-eight implicated genes, including two known drivers of human melanoma, were classified into three groups based on high, low, or background-level mutation frequencies in human melanomas, and we further explored the functional significance of genes in each group. For two genes overlooked by prevailing discovery methods, we fou","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Sep","modification":"2026-04-13T17:59:34.747Z","creation":"2026-04-07T13:36:26.178Z"},"accession":"S-EPMC3780872","cross_references":{"pubmed":["24003131"],"doi":["10.1073/pnas.1314435110"]}}