biostudies-literatureLeymarie NNIDCR NIH HHSNCRR NIH HHSNIAID NIH HHSNHLBI NIH HHSNIGMS NIH HHSPHS HHSNIH HHS2935-51https://www.ebi.ac.uk/biostudies/studies/S-EPMC3790302biostudies-literatureUnknown12(10)One of the principal goals of glycoprotein research is to correlate glycan structure and function. Such correlation is necessary in order for one to understand the mechanisms whereby glycoprotein structure elaborates the functions of myriad proteins. The accurate comparison of glycoforms and quantification of glycosites are essential steps in this direction. Mass spectrometry has emerged as a powerful analytical technique in the field of glycoprotein characterization. Its sensitivity, high dynamic range, and mass accuracy provide both quantitative and sequence/structural information. As part of the 2012 ABRF Glycoprotein Research Group study, we explored the use of mass spectrometry and ancillary methodologies to characterize the glycoforms of two sources of human prostate specific antigenMolecular & cellular proteomics : MCPInterlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012.PMC3790302S10 RR 020946S10 OD010731R01 AI094797R01 GM103547S10 RR 015942S10 RR 025082S10 RR020946R01 098050P41 P41GM104603N01 HV 00239S10 RR025082R01 DE020925P41 GM104603Horn DMWatson MJonscher KWuhrer MBrown KJZaia JAguilan JResemann AWhittal RWada YGoldman RBall LENyalwidhe JOSchweiger-Hufnagel UMeyer BMcComb MMoginger USchulz JMWindwarder MBasumallick LRohrer JSBehnken HAlley WRCairo CWLy MDaneshfar RSanda MCipollo JFPompach PHurum DZhu YDesaire HNilsson JReiz BZou CTang HBazemore-Walker CRHalim AKolarich DJabs WMarx KValmu LGo EPBunz SCThaysen-Andersen MAn YNeusuβ CDrake RRGruber CSihlbom CGriffin PJPacker NHViner RINovotny MVLeymarie NSong EMechref YHensbergen PJRuthenbeck ABlank MAStaples GOHathout YMann BFLarson GOrlando RSuckau DAltmann FfalseInterlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012.One of the principal goals of glycoprotein research is to correlate glycan structure and function. Such correlation is necessary in order for one to understand the mechanisms whereby glycoprotein structure elaborates the functions of myriad proteins. The accurate comparison of glycoforms and quantification of glycosites are essential steps in this direction. Mass spectrometry has emerged as a powerful analytical technique in the field of glycoprotein characterization. Its sensitivity, high dynamic range, and mass accuracy provide both quantitative and sequence/structural information. As part of the 2012 ABRF Glycoprotein Research Group study, we explored the use of mass spectrometry and ancillary methodologies to characterize the glycoforms of two sources of human prostate specific antigen2013-01-01T00:00:00Z2013 Oct2026-05-01T15:13:37.485Z2026-04-07T16:59:56.18ZS-EPMC37903022376450210.1074/mcp.m113.03064310.1074/mcp.M113.030643