biostudies-literatureMoscovitch-Lopatin MNHGRI NIH HHSNINDS NIH HHS1134-40https://www.ebi.ac.uk/biostudies/studies/S-EPMC3795598biostudies-literatureUnknown81(13)<h4>Objective</h4>We measured the levels of mutant huntingtin (mtHtt) and total huntingtin (tHtt) in blood leukocytes from Prospective Huntington At-Risk Observational Study (PHAROS) subjects at 50% risk of carrying the Huntington disease mutation using a homogeneous time-resolved fluorescence (HTRF) assay to assess its potential as a biomarker.<h4>Methods</h4>Peripheral blood mononuclear cells from consenting PHAROS subjects were analyzed by HTRF using antibodies that simultaneously measured mtHtt and tHtt. mtHtt levels were normalized to tHtt, double-stranded DNA, or protein and analyzed according to cytosine-adenine-guanine repeat length (CAGn), demographics, predicted time to clinical onset or known time since clinical onset, and available clinical measures.<h4>Results</h4>From 363 assNeurologyHTRF analysis of soluble huntingtin in PHAROS PBMCs.PMC3795598UO1NS071789P01NS058793HG02449-01U01 NS071789Goodman REMatson SMoscovitch-Lopatin MShoulson IMatson WRosas HDYoung ABHersch SMHuntington Study Group PHAROS InvestigatorsEberly SRitch JJOakes DfalseHTRF analysis of soluble huntingtin in PHAROS PBMCs.<h4>Objective</h4>We measured the levels of mutant huntingtin (mtHtt) and total huntingtin (tHtt) in blood leukocytes from Prospective Huntington At-Risk Observational Study (PHAROS) subjects at 50% risk of carrying the Huntington disease mutation using a homogeneous time-resolved fluorescence (HTRF) assay to assess its potential as a biomarker.<h4>Methods</h4>Peripheral blood mononuclear cells from consenting PHAROS subjects were analyzed by HTRF using antibodies that simultaneously measured mtHtt and tHtt. mtHtt levels were normalized to tHtt, double-stranded DNA, or protein and analyzed according to cytosine-adenine-guanine repeat length (CAGn), demographics, predicted time to clinical onset or known time since clinical onset, and available clinical measures.<h4>Results</h4>From 363 ass2013-01-01T00:00:00Z2013 Sep2026-05-02T09:38:30.503Z2019-03-27T01:17:08ZS-EPMC37955982396624710.1212/WNL.0b013e3182a55ede10.1212/wnl.0b013e3182a55ede