{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Alcantar-Orozco EM"],"funding":["Cancer Research UK","Medical Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["310-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3798231"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(5)"],"pubmed_abstract":["The genetic modification of peripheral blood lymphocytes using retroviral vectors to redirect T cells against tumor cells has been recently used as a means to generate large numbers of antigen-specific T cells for adoptive cell therapy protocols. However, commonly used retroviral vector-based genetic modification requires T cells to be driven into cell division; this potent mitogenic stimulus is associated with the development of an effector phenotype that may adversely impact upon the long-term engraftment potential and subsequent antitumor effects of T cells. To investigate whether the cytokines used during culture impact upon the engraftment potential of gene-modified T cells, a humanized model employing T cells engrafted with a MART-1-specific T cell receptor adoptively transferred int"],"journal":["Human gene therapy methods"],"pubmed_title":["Potential limitations of the NSG humanized mouse as a model system to optimize engineered human T cell therapy for cancer."],"pmcid":["PMC3798231"],"funding_grant_id":["MR/K500732/1"],"pubmed_authors":["Gornall H","Alcantar-Orozco EM","Baldan V","Hawkins RE","Gilham DE"],"additional_accession":[]},"is_claimable":false,"name":"Potential limitations of the NSG humanized mouse as a model system to optimize engineered human T cell therapy for cancer.","description":"The genetic modification of peripheral blood lymphocytes using retroviral vectors to redirect T cells against tumor cells has been recently used as a means to generate large numbers of antigen-specific T cells for adoptive cell therapy protocols. However, commonly used retroviral vector-based genetic modification requires T cells to be driven into cell division; this potent mitogenic stimulus is associated with the development of an effector phenotype that may adversely impact upon the long-term engraftment potential and subsequent antitumor effects of T cells. To investigate whether the cytokines used during culture impact upon the engraftment potential of gene-modified T cells, a humanized model employing T cells engrafted with a MART-1-specific T cell receptor adoptively transferred int","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Oct","modification":"2025-04-26T13:59:04.155Z","creation":"2019-03-27T01:17:14Z"},"accession":"S-EPMC3798231","cross_references":{"pubmed":["23931270"],"doi":["10.1089/hgtb.2013.022"]}}