biostudies-literatureAlcantar-Orozco EMCancer Research UKMedical Research CouncilBiotechnology and Biological Sciences Research Council310-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC3798231biostudies-literatureUnknown24(5)The genetic modification of peripheral blood lymphocytes using retroviral vectors to redirect T cells against tumor cells has been recently used as a means to generate large numbers of antigen-specific T cells for adoptive cell therapy protocols. However, commonly used retroviral vector-based genetic modification requires T cells to be driven into cell division; this potent mitogenic stimulus is associated with the development of an effector phenotype that may adversely impact upon the long-term engraftment potential and subsequent antitumor effects of T cells. To investigate whether the cytokines used during culture impact upon the engraftment potential of gene-modified T cells, a humanized model employing T cells engrafted with a MART-1-specific T cell receptor adoptively transferred intHuman gene therapy methodsPotential limitations of the NSG humanized mouse as a model system to optimize engineered human T cell therapy for cancer.PMC3798231MR/K500732/1Gornall HAlcantar-Orozco EMBaldan VHawkins REGilham DEfalsePotential limitations of the NSG humanized mouse as a model system to optimize engineered human T cell therapy for cancer.The genetic modification of peripheral blood lymphocytes using retroviral vectors to redirect T cells against tumor cells has been recently used as a means to generate large numbers of antigen-specific T cells for adoptive cell therapy protocols. However, commonly used retroviral vector-based genetic modification requires T cells to be driven into cell division; this potent mitogenic stimulus is associated with the development of an effector phenotype that may adversely impact upon the long-term engraftment potential and subsequent antitumor effects of T cells. To investigate whether the cytokines used during culture impact upon the engraftment potential of gene-modified T cells, a humanized model employing T cells engrafted with a MART-1-specific T cell receptor adoptively transferred int2013-01-01T00:00:00Z2013 Oct2025-04-26T13:59:04.155Z2019-03-27T01:17:14ZS-EPMC37982312393127010.1089/hgtb.2013.022