biostudies-literatureUnknown3Caramuta SDeregulation of microRNA (miRNA) expression has been documented in diffuse large B-cell lymphoma (DLBCL). However, the impact of miRNAs and their machinery in DLBCL is not fully determined. Here, we assessed the role of miRNA expression and their processing genes in DLBCL development. Using microarray and RT-qPCR approaches, we quantified global miRNAs and core components of miRNA-processing genes expression in 75 DLBCLs (56 de novo and 19 transformed) and 10 lymph nodes (LN). Differential miRNA signatures were identified between DLBCLs and LNs, or between the de novo and transformed DLBCLs. We also identified subsets of miRNAs associated with germinal center B-cell phenotype, BCL6 and IRF4 expression, and clinical staging. In addition, we showed a significant over-expression of TARBP2 in Blood cancer journale152https://www.ebi.ac.uk/biostudies/studies/S-EPMC3816210biostudies-literatureRole of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma.PMC3816210Akcakaya PLarsson CBerglund MCaramuta SXie HEnblad GLee LLui WOOzata DMGeorgii-Hemming PAmini RMLawrie CHfalseRole of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma.Deregulation of microRNA (miRNA) expression has been documented in diffuse large B-cell lymphoma (DLBCL). However, the impact of miRNAs and their machinery in DLBCL is not fully determined. Here, we assessed the role of miRNA expression and their processing genes in DLBCL development. Using microarray and RT-qPCR approaches, we quantified global miRNAs and core components of miRNA-processing genes expression in 75 DLBCLs (56 de novo and 19 transformed) and 10 lymph nodes (LN). Differential miRNA signatures were identified between DLBCLs and LNs, or between the de novo and transformed DLBCLs. We also identified subsets of miRNAs associated with germinal center B-cell phenotype, BCL6 and IRF4 expression, and clinical staging. In addition, we showed a significant over-expression of TARBP2 in 2013-01-01T00:00:00Z2013 Oct2026-05-04T12:22:40.803Z2026-04-07T20:33:27.873ZS-EPMC38162102412116410.1038/bcj.2013.49