{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chai S"],"funding":["NIMH NIH HHS","NINDS NIH HHS"],"pagination":["17898-903"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3816481"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["110(44)"],"pubmed_abstract":["MicroRNA-134 (miR-134) serves as a widely accepted model for microRNA function in synaptic plasticity. In this model, synaptic activity stimulates miR-134 expression, which then regulates dendrite growth and spine formation. By using a ratiometric microRNA sensor, we found, unexpectedly, that miR-134 activity in cortical neurons was restricted to interneurons. Using an assay designed to trap microRNA-mRNA complexes, we determined that miR-134 interacted directly with the mRNA encoding the palmitoylation enzyme, DHHC9. This enzyme is known to palmitoylate H-Ras, a modification required for proper membrane trafficking. Treatment with bicuculline, a GABAA receptor antagonist, decreased DHHC9 expression in somatostatin-positive interneurons and membrane localization of an H-Ras reporter in a m"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["MicroRNA-134 activity in somatostatin interneurons regulates H-Ras localization by repressing the palmitoylation enzyme, DHHC9."],"pmcid":["PMC3816481"],"funding_grant_id":["NS097317","NS076094","MH099099","P30 NS069305","F32 NS076094","R01 MH099099"],"pubmed_authors":["Eichhorn SW","Chai S","Cambronne XA","Goodman RH"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-134 activity in somatostatin interneurons regulates H-Ras localization by repressing the palmitoylation enzyme, DHHC9.","description":"MicroRNA-134 (miR-134) serves as a widely accepted model for microRNA function in synaptic plasticity. In this model, synaptic activity stimulates miR-134 expression, which then regulates dendrite growth and spine formation. By using a ratiometric microRNA sensor, we found, unexpectedly, that miR-134 activity in cortical neurons was restricted to interneurons. Using an assay designed to trap microRNA-mRNA complexes, we determined that miR-134 interacted directly with the mRNA encoding the palmitoylation enzyme, DHHC9. This enzyme is known to palmitoylate H-Ras, a modification required for proper membrane trafficking. Treatment with bicuculline, a GABAA receptor antagonist, decreased DHHC9 expression in somatostatin-positive interneurons and membrane localization of an H-Ras reporter in a m","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Oct","modification":"2025-04-04T12:47:25.371Z","creation":"2019-03-27T01:18:04Z"},"accession":"S-EPMC3816481","cross_references":{"pubmed":["24127608"],"doi":["10.1073/pnas.1317528110"]}}