biostudies-literatureKefas BNCI NIH HHS1652-63https://www.ebi.ac.uk/biostudies/studies/S-EPMC3829588biostudies-literatureUnknown15(12)<h4>Background</h4>Despite advances in the treatment of the most aggressive form of brain tumor, glioblastoma, patient prognosis remains disappointing. This failure in treatment has been attributed to dysregulated oncogenic pathways, as observed in other tumors. We and others have suggested the use of microRNAs (miRs) as therapeutic tools able to target multiple pathways in glioblastoma.<h4>Methods</h4>This work features PCR quantification of miRs and transient transfection of many glioblastoma cell lines with miRs, followed by cell number analysis, trypan blue staining, alamarBlue assay of cell viability, caspase-3/-7 activity assay, immunoblot of cleaved poly(ADP-ribose) polymerase and fluorescence activated cell sorting and imaging of apoptotic nuclei, cell invasion assays, MRIs of glioNeuro-oncologyA miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.PMC3829588R01CA136803P30 CA044579Frisbee AAbounader RPurow BFloyd DHGuessous FKefas BDominguez CComeau LDipierro CGfalseA miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.<h4>Background</h4>Despite advances in the treatment of the most aggressive form of brain tumor, glioblastoma, patient prognosis remains disappointing. This failure in treatment has been attributed to dysregulated oncogenic pathways, as observed in other tumors. We and others have suggested the use of microRNAs (miRs) as therapeutic tools able to target multiple pathways in glioblastoma.<h4>Methods</h4>This work features PCR quantification of miRs and transient transfection of many glioblastoma cell lines with miRs, followed by cell number analysis, trypan blue staining, alamarBlue assay of cell viability, caspase-3/-7 activity assay, immunoblot of cleaved poly(ADP-ribose) polymerase and fluorescence activated cell sorting and imaging of apoptotic nuclei, cell invasion assays, MRIs of glio2013-01-01T00:00:00Z2013 Dec2025-04-05T14:23:24.624Z2019-03-27T03:08:39ZS-EPMC38295882415811110.1093/neuonc/not118