biostudies-literatureUnknown12Canier L<h4>Background</h4>To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field.<h4>Methods</h4>DNA extraction and real-time PCR assays were implemented in an "in-house" designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831).<h4>Results</h4>The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in RaMalaria journal405https://www.ebi.ac.uk/biostudies/studies/S-EPMC3829804biostudies-literatureAn innovative tool for moving malaria PCR detection of parasite reservoir into the field.PMC3829804Uk SSokny MDurnez LCanier LMasse-Navette PSluydts VLoch KMenard DHeng SEam RChuor CMCoosemans MTho SDourng DGryseels CVan Roey KChy SKhim NDeubel VThavrin BKim SKhean CSiv SKen MGrietens KPLim HfalseAn innovative tool for moving malaria PCR detection of parasite reservoir into the field.<h4>Background</h4>To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field.<h4>Methods</h4>DNA extraction and real-time PCR assays were implemented in an "in-house" designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831).<h4>Results</h4>The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Ra2013-01-01T00:00:00Z2013 Nov2026-07-15T05:23:43.733Z2026-06-30T03:22:59.072ZS-EPMC38298042420664910.1186/1475-2875-12-405