biostudies-literatureUnknown70(4)Hua QStudies of steady-state metabolic fluxes in Escherichia coli grown in nutrient-limited chemostat cultures suggest remarkable flux alterations in response to changes of growth-limiting nutrient in the medium (Hua et al., J. Bacteriol. 185:7053-7067, 2003). To elucidate the physiological adaptation of cells to the nutrient condition through the flux change and understand the molecular mechanisms underlying the change in the flux, information on gene expression is of great importance. DNA microarray analysis was performed to investigate the global transcriptional responses of steady-state cells grown in chemostat cultures with limited glucose or ammonia while other environmental conditions and the growth rate were kept constant. In slow-growing cells (specific growth rate of 0.10 h(-1)), 9.8%Applied and environmental microbiology2354-66https://www.ebi.ac.uk/biostudies/studies/S-EPMC383082biostudies-literatureAnalysis of gene expression in Escherichia coli in response to changes of growth-limiting nutrient in chemostat cultures.PMC383082Oshima TMori HHua QYang CShimizu KfalseAnalysis of gene expression in Escherichia coli in response to changes of growth-limiting nutrient in chemostat cultures.Studies of steady-state metabolic fluxes in Escherichia coli grown in nutrient-limited chemostat cultures suggest remarkable flux alterations in response to changes of growth-limiting nutrient in the medium (Hua et al., J. Bacteriol. 185:7053-7067, 2003). To elucidate the physiological adaptation of cells to the nutrient condition through the flux change and understand the molecular mechanisms underlying the change in the flux, information on gene expression is of great importance. DNA microarray analysis was performed to investigate the global transcriptional responses of steady-state cells grown in chemostat cultures with limited glucose or ammonia while other environmental conditions and the growth rate were kept constant. In slow-growing cells (specific growth rate of 0.10 h(-1)), 9.8%2004-01-01T00:00:00Z2004 Apr2025-04-18T16:45:51.327Z2025-04-07T04:09:15.81ZS-EPMC3830821506683210.1128/aem.70.4.2354-2366.200410.1128/AEM.70.4.2354-2366.2004