{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(12)"],"submitter":["Culot M"],"pubmed_abstract":["Historically, the focus has been to use in vitro BBB models to optimize rate of drug delivery to the CNS, whereas total in vivo brain/plasma ratios have been used for optimizing extent. However, these two parameters do not necessarily show good correlations with receptor occupancy data or other pharmacological readouts. In line with the free drug hypothesis, the use of unbound brain concentrations (Cu,br) has been shown to provide the best correlations with pharmacological data. However, typically the determination of this parameter requires microdialysis, a technique not ideally suited for screening in early drug development. Alternative, and less resource-demanding methodologies to determine Cu,br employ either equilibrium dialysis of brain homogenates or incubations of brain slices in b"],"journal":["PloS one"],"pagination":["e80634"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3849192"],"repository":["biostudies-literature"],"pubmed_title":["A simple method for assessing free brain/free plasma ratios using an in vitro model of the blood brain barrier."],"pmcid":["PMC3849192"],"pubmed_authors":["Sevin E","Culot M","Martinsson S","Szorath E","Cecchelli R","Hongmei Y","Renftel M","Lundquist S","Fabulas-da Costa A"],"additional_accession":[]},"is_claimable":false,"name":"A simple method for assessing free brain/free plasma ratios using an in vitro model of the blood brain barrier.","description":"Historically, the focus has been to use in vitro BBB models to optimize rate of drug delivery to the CNS, whereas total in vivo brain/plasma ratios have been used for optimizing extent. However, these two parameters do not necessarily show good correlations with receptor occupancy data or other pharmacological readouts. In line with the free drug hypothesis, the use of unbound brain concentrations (Cu,br) has been shown to provide the best correlations with pharmacological data. However, typically the determination of this parameter requires microdialysis, a technique not ideally suited for screening in early drug development. Alternative, and less resource-demanding methodologies to determine Cu,br employ either equilibrium dialysis of brain homogenates or incubations of brain slices in b","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-05-29T19:37:06.268Z","creation":"2025-05-29T19:37:06.268Z"},"accession":"S-EPMC3849192","cross_references":{"pubmed":["24312489"],"doi":["10.1371/journal.pone.0080634"]}}