{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(12)"],"submitter":["Meyrand M"],"pubmed_abstract":["Surface proteins of Gram-positive bacteria play crucial roles in bacterial adhesion to host tissues. Regarding commensal or probiotic bacteria, adhesion to intestinal mucosa may promote their persistence in the gastro-intestinal tract and their beneficial effects to the host. In this study, seven Lactococcus lactis strains exhibiting variable surface physico-chemical properties were compared for their adhesion to Caco-2 intestinal epithelial cells. In this test, only one vegetal isolate TIL448 expressed a high-adhesion phenotype. A nonadhesive derivative was obtained by plasmid curing from TIL448, indicating that the adhesion determinants were plasmid-encoded. Surface-exposed proteins in TIL448 were analyzed by a proteomic approach consisting in shaving of the bacterial surface with trypsi"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["3935-47"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3861735"],"repository":["biostudies-literature"],"pubmed_title":["Surface proteome analysis of a natural isolate of Lactococcus lactis reveals the presence of pili able to bind human intestinal epithelial cells."],"pmcid":["PMC3861735"],"pubmed_authors":["Trugnan G","Meyrand M","Guillot A","Chapot-Chartier MP","Thomas G","Kulakauskas S","Cortes-Perez NG","Dupres V","Armalyte J","Goin M","Dufrene YF","Hols P","Pechoux C","Chat S","Furlan S"],"additional_accession":[]},"is_claimable":false,"name":"Surface proteome analysis of a natural isolate of Lactococcus lactis reveals the presence of pili able to bind human intestinal epithelial cells.","description":"Surface proteins of Gram-positive bacteria play crucial roles in bacterial adhesion to host tissues. Regarding commensal or probiotic bacteria, adhesion to intestinal mucosa may promote their persistence in the gastro-intestinal tract and their beneficial effects to the host. In this study, seven Lactococcus lactis strains exhibiting variable surface physico-chemical properties were compared for their adhesion to Caco-2 intestinal epithelial cells. In this test, only one vegetal isolate TIL448 expressed a high-adhesion phenotype. A nonadhesive derivative was obtained by plasmid curing from TIL448, indicating that the adhesion determinants were plasmid-encoded. Surface-exposed proteins in TIL448 were analyzed by a proteomic approach consisting in shaving of the bacterial surface with trypsi","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Dec","modification":"2026-05-04T05:22:12.775Z","creation":"2026-04-07T20:07:44.66Z"},"accession":"S-EPMC3861735","cross_references":{"pubmed":["24002364"],"doi":["10.1074/mcp.M113.029066","10.1074/mcp.m113.029066"]}}