biostudies-literatureUnknown8(12)Maheandiran MIt is well accepted that insulin-induced hypoglycemia can result in seizures. However, the effects of the seizures, as well as possible treatment strategies, have yet to be elucidated, particularly in juvenile or insulin-dependent diabetes mellitus (IDDM). Here we establish a model of diabetes in young rats, to examine the consequences of severe hypoglycemia in this age group; particularly seizures and mortality. Diabetes was induced in post-weaned 22-day-old Sprague-Dawley rats by streptozotocin (STZ) administered intraperitoneally (IP). Insulin IP (15 U/kg), in rats fasted (14-16 hours), induced hypoglycemia, defined as <3.5 mM blood glucose (BG), in 68% of diabetic (STZ) and 86% of control rats (CON). Seizures occurred in 86% of STZ and all CON rats that reached hypoglycemic levels withPloS onee83168https://www.ebi.ac.uk/biostudies/studies/S-EPMC3875447biostudies-literatureSevere hypoglycemia in a juvenile diabetic rat model: presence and severity of seizures are associated with mortality.PMC3875447Sugumar SMaheandiran MCarlen PLdel Campo MEl-Hayek YWu CGiacca AMylvaganam SZhang LHazrati LfalseSevere hypoglycemia in a juvenile diabetic rat model: presence and severity of seizures are associated with mortality.It is well accepted that insulin-induced hypoglycemia can result in seizures. However, the effects of the seizures, as well as possible treatment strategies, have yet to be elucidated, particularly in juvenile or insulin-dependent diabetes mellitus (IDDM). Here we establish a model of diabetes in young rats, to examine the consequences of severe hypoglycemia in this age group; particularly seizures and mortality. Diabetes was induced in post-weaned 22-day-old Sprague-Dawley rats by streptozotocin (STZ) administered intraperitoneally (IP). Insulin IP (15 U/kg), in rats fasted (14-16 hours), induced hypoglycemia, defined as <3.5 mM blood glucose (BG), in 68% of diabetic (STZ) and 86% of control rats (CON). Seizures occurred in 86% of STZ and all CON rats that reached hypoglycemic levels with2013-01-01T00:00:00Z20132025-04-26T09:59:51.779Z2019-03-26T23:21:20ZS-EPMC38754472438615610.1371/journal.pone.0083168