biostudies-literatureUnknown11(1)Ye P<h4>Objective</h4>miR-126, the miRNA considered to be specially expressed in endothelial cells and hematopoietic progenitor cells, is strongly associated with angiogenesis. The purpose is to evaluate the role of miR-126 in hypoxia-induced angiogenesis and the possible mechanisms.<h4>Methods</h4>The expression of miR-126 was detected in hypoxia-treated RF/6A cells and diabetic retinas using real-time PCR. The miR-126 was up- or down-regulated by transfecting miR-126-mimics or inhibitors into RF/6A cells. Cell cycle analysis was performed using flow cytometry. The protein levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were assessed by immunoblotting.<h4>Results</h4>A significantly decreased expression of miR-126 was found in hypoxia-treated RF/6A cInternational journal of medical sciences17-23https://www.ebi.ac.uk/biostudies/studies/S-EPMC3880987biostudies-literatureHypoxia-induced deregulation of miR-126 and its regulative effect on VEGF and MMP-9 expression.PMC3880987He FYao KLiu JYe PXu WfalseHypoxia-induced deregulation of miR-126 and its regulative effect on VEGF and MMP-9 expression.<h4>Objective</h4>miR-126, the miRNA considered to be specially expressed in endothelial cells and hematopoietic progenitor cells, is strongly associated with angiogenesis. The purpose is to evaluate the role of miR-126 in hypoxia-induced angiogenesis and the possible mechanisms.<h4>Methods</h4>The expression of miR-126 was detected in hypoxia-treated RF/6A cells and diabetic retinas using real-time PCR. The miR-126 was up- or down-regulated by transfecting miR-126-mimics or inhibitors into RF/6A cells. Cell cycle analysis was performed using flow cytometry. The protein levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were assessed by immunoblotting.<h4>Results</h4>A significantly decreased expression of miR-126 was found in hypoxia-treated RF/6A c2014-01-01T00:00:00Z20142025-04-18T18:18:18.621Z2019-03-27T01:19:25ZS-EPMC38809872439628210.7150/ijms.7329