{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Ding Z"],"pubmed_abstract":["<h4>Background</h4>Affinity precipitation has been reported as a potential technology for the purification of proteins at the early stage of downstream processing. The technology could be achieved using reversible soluble-insoluble polymers coupled with an affinity ligand to purify proteins from large volumes of dilute solution material such as fermentation broths or plasma. In this study, a thermo-response polymer was synthesized using N-methylol acrylamide, N-isopropyl acrylamide and butyl acrylate as monomers. The molecular weight of the polymer measured by the viscosity method was 3.06 × 104 Da and the lower critical solution temperature (LCST) was 28.0°C.The recovery of the polymer above the LCST was over 95.0%. Human serum albumin (HSA) is the most abundant protein in the human serum"],"journal":["BMC biotechnology"],"pagination":["109"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3903448"],"repository":["biostudies-literature"],"pubmed_title":["Affinity precipitation of human serum albumin using a thermo-response polymer with an L-thyroxin ligand."],"pmcid":["PMC3903448"],"pubmed_authors":["Cao X","Ding Z"],"additional_accession":[]},"is_claimable":false,"name":"Affinity precipitation of human serum albumin using a thermo-response polymer with an L-thyroxin ligand.","description":"<h4>Background</h4>Affinity precipitation has been reported as a potential technology for the purification of proteins at the early stage of downstream processing. The technology could be achieved using reversible soluble-insoluble polymers coupled with an affinity ligand to purify proteins from large volumes of dilute solution material such as fermentation broths or plasma. In this study, a thermo-response polymer was synthesized using N-methylol acrylamide, N-isopropyl acrylamide and butyl acrylate as monomers. The molecular weight of the polymer measured by the viscosity method was 3.06 × 104 Da and the lower critical solution temperature (LCST) was 28.0°C.The recovery of the polymer above the LCST was over 95.0%. Human serum albumin (HSA) is the most abundant protein in the human serum","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Dec","modification":"2025-04-21T20:00:31.424Z","creation":"2019-03-27T01:20:42Z"},"accession":"S-EPMC3903448","cross_references":{"pubmed":["24341315"],"doi":["10.1186/1472-6750-13-109"]}}